Objective Development of a safe and effective vaccine against human respiratory syncytial virus, and its close bovine relative, remains a high priority. These viruses are the most important cause of severe respiratory infections in very young children and calves. We intend to use recently developed techniques for rescuing negative- stranded RNA viruses from cDNA copies of the entire genome. This approach offers the possibility of using highly developed genetic engineering techniques for introducing desired mutations in the viral genome. Minigenome amplication and alternative assays will be used for rapid screening of promising mutations that may be then transferred to full-length cDNA clones for virus rescue. Mutant viruses will be tested in cell and organ cultures for growth and induction of key regulatory molecules. Viruses with interesting phenotypes will be tested in animal models, including calves as a natural host of bovine respiratory syncytial virus. Fields of science medical and health scienceshealth sciencesinfectious diseasesRNA virusesnatural sciencesbiological sciencesmicrobiologyvirologymedical and health sciencesmedical biotechnologygenetic engineeringmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsvaccinesnatural sciencesbiological sciencesgeneticsmutation Programme(s) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Topic(s) 1.1.1.-2. - Key action Control of Infectious Diseases Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator INSTITUTE OF HEALTH CARLOS III EU contribution No data Address Carretera de Majadahonda a Pozuelo 28220 MAJADAHONDA Spain See on map Total cost No data Participants (4) Sort alphabetically Sort by EU Contribution Expand all Collapse all INSTITUTE FOR ANIMAL HEALTH United Kingdom EU contribution No data Address Compton Laboratory RG20 7NN NEWBURY See on map Total cost No data LUDWIG-MAXIMILIANS UNIVERSITY OF MUNICH Germany EU contribution No data Address Geschwister-Scholl-Platz 1 80539 MUENCHEN See on map Total cost No data SCHOOL OF VETERINARY MEDICINE, HANNOVER Germany EU contribution No data Address Buenteweg 17 30559 HANNOVER See on map Total cost No data UNIVERSITY OF WARWICK United Kingdom EU contribution No data Address GIBBET HILL ROAD COVENTRY See on map Links Website Opens in new window Total cost No data