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Fungal metalloenzimes oxidizing aromatic compounds of industrial interest

Objective

The first objective is to overcome one of the main bottlenecks for the use of laccases and peroxidases from ligninolytic basidiomycetes metalloenzymes with high technological potential because of ability to oxidize aromatic compounds, which is related to the difficulties to have high amount of active enzyme. Different strategies for their heterologous expression will be intended including optimised refolding of protein from bacterial inclusion bodies, improved production and secretion using industrial ascomycete and development of a new basidiomycete expression system. Simultaneously, several key aspects of their catalytic activity will be investigated using techniques for protein structural characterization (x-ray and NMR) and confirmed by site-directed mutagenesis. With the above information in hand tailor-made enzymes will be designed to improved (catalytic efficiency and/or stability) and produced using the best expression systems. Finally, fermentation technology for production of native and engineered enzymes will be optimised, and in dust. Applications evaluated with the collaboration of two enzyme-product.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Address
144,Velazquez 144
28006 Madrid
Spain

Participants (8)

BELDEM S.A.
Belgium
Address
Rue Bourrie 12
5300 Andenne,seilles
Institut National de la Recherche Agronomique (INRA)
France
Address
147 Rue De L'université
75341 Paris
NETHERLANDS RESEARCH SCHOOL FOR ASTRONOMY - LEGALLY REPRESENTED BY UNIVERSITY OF GRONINGEN
Netherlands
Address
5,Broerstraat 5
9712 CP Groningen
NOVO-NORDISK A/S
Denmark
Address
1,Novo Allt, 6B3
2880 Bagsvaerd
SWISS FEDERAL INSTITUTE OF TECHNOLOGY ZUERICH
Switzerland
Address
101,Raemistrasse 101, Eth Zentrum
8092 Zurich
UNIVERSITY OF FLORENCE
Italy
Address
Piazza San Marco 4
50121 Firenze
UNIVERSITY OF HELSINKI
Finland
Address
Yliopistonkatu 4
00014 Helsinki
UNIVERSITY OF SUSSEX
United Kingdom
Address
Falmer
BN1 9QG Brighton