The first objective is to overcome one of the main bottlenecks for the use of laccases and peroxidases from ligninolytic basidiomycetes metalloenzymes with high technological potential because of ability to oxidize aromatic compounds, which is related to the difficulties to have high amount of active enzyme. Different strategies for their heterologous expression will be intended including optimised refolding of protein from bacterial inclusion bodies, improved production and secretion using industrial ascomycete and development of a new basidiomycete expression system. Simultaneously, several key aspects of their catalytic activity will be investigated using techniques for protein structural characterization (x-ray and NMR) and confirmed by site-directed mutagenesis. With the above information in hand tailor-made enzymes will be designed to improved (catalytic efficiency and/or stability) and produced using the best expression systems. Finally, fermentation technology for production of native and engineered enzymes will be optimised, and in dust. Applications evaluated with the collaboration of two enzyme-product.
Funding SchemeCSC - Cost-sharing contracts
9712 CP Groningen
BN1 9QG Brighton