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Fungal metalloenzimes oxidizing aromatic compounds of industrial interest

Fungal metalloenzimes oxidizing aromatic compounds of industrial interest

Objective

The first objective is to overcome one of the main bottlenecks for the use of laccases and peroxidases from ligninolytic basidiomycetes metalloenzymes with high technological potential because of ability to oxidize aromatic compounds, which is related to the difficulties to have high amount of active enzyme. Different strategies for their heterologous expression will be intended including optimised refolding of protein from bacterial inclusion bodies, improved production and secretion using industrial ascomycete and development of a new basidiomycete expression system. Simultaneously, several key aspects of their catalytic activity will be investigated using techniques for protein structural characterization (x-ray and NMR) and confirmed by site-directed mutagenesis. With the above information in hand tailor-made enzymes will be designed to improved (catalytic efficiency and/or stability) and produced using the best expression systems. Finally, fermentation technology for production of native and engineered enzymes will be optimised, and in dust. Applications evaluated with the collaboration of two enzyme-product.

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Coordinator

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

Address

144,Velazquez 144
28006 Madrid

Spain

Administrative Contact

César NOMBELA (Dr.)

Participants (8)

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BELDEM S.A.

Belgium

Institut National de la Recherche Agronomique (INRA)

France

NETHERLANDS RESEARCH SCHOOL FOR ASTRONOMY - LEGALLY REPRESENTED BY UNIVERSITY OF GRONINGEN

Netherlands

NOVO-NORDISK A/S

Denmark

SWISS FEDERAL INSTITUTE OF TECHNOLOGY ZUERICH

Switzerland

UNIVERSITY OF FLORENCE

Italy

UNIVERSITY OF HELSINKI

Finland

UNIVERSITY OF SUSSEX

United Kingdom

Project information

Grant agreement ID: QLK3-CT-1999-00590

  • Start date

    1 February 2000

  • End date

    1 August 2003

Funded under:

FP5-LIFE QUALITY

  • Overall budget:

    € 2 420 970

  • EU contribution

    € 1 394 600

Coordinated by:

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

Spain