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Regulatory gene initiative in arabidopsis

Regulatory gene initiative in arabidopsis

Objective

Transcription factors (TF) are recognised as having a great biotechnological potential and also to have played a pivotal role in the evolution of plants.

The aim of this project is to establish the function of plant TFs, and it will involve:
(i)the definition of TF gene expression patterns in Arabidopsis;
(ii) the identification of insertion mutations at TF loci;
(iii) the ectopic expression of TFs (or derivatives) in Arabidopsis and in crop plants;
(iv) their phenotypic analysis;
(v) the analysis of interactions between TFs, and
(vi) the generation of a bio-informatics infrastructure.

This programme will help to reveal the full biotechnological potential of plant TFs and provide insights into hierarchies, redundancies, interdependencies and into their evolution. The study will involve the preparation of a TF gene array and of a normalised TF ORF library in a 2-Hybrid vector, two tasks justifying the European dimension of the project.
Second edition of the TF gene array, containing 1194 probes and 26 controls..81 Arabidopsis lines containing constructs over-expressing TFs and 28 RNA interference/antisense RNA constructs.·7 tomato lines transformed with TF over-expressors, RNAi or antisense constructs. ·4 rapeseed lines transformed with TF over-expressors, RNAi or antisense constructs. ·Improved methods for high-throughput metabolic and AFLP-based RNA profiling. ·Library of 125 insertion mutants at TF loci. ·Web accessible database bringing together information on TFs, their gene sequences, and interactions, with custom-built navigation and interrogation tools.
Leaflet | Map data © OpenStreetMap contributors, Credit: EC-GISCO, © EuroGeographics for the administrative boundaries

Coordinator

AMICA SCIENCE EEIG

Address

Norwich Research Park John Innes Centre, Colney
Nr4 7uh Norwich

United Kingdom

Administrative Contact

Donald O'NIONS (Mr.)

Participants (22)

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ALBERT-LUDWIGS-UNIVERSITAET FREIBURG

Germany

BASF PLANT SCIENCE GMBH

Germany

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

France

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

Spain

FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY VZW

Belgium

GEORG-AUGUST-UNIVERSITY GOETTINGEN

Germany

INSTITUTE OF PLANT GENETICS AND CROP PLANT RESEARCH

Germany

Institut National de la Recherche Agronomique (INRA)

France

JOHN INNES CENTRE

United Kingdom

MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.

Germany

METANOMICS GMBH & KOKGAA

Germany

NATIONAL RESEARCH COUNCIL OF ITALY

Italy

PLANT RESEARCH INTERNATIONAL B.V.

Netherlands

SYNGENTA LIMITED

United Kingdom

THE PROVOST, FELLOWS AND SCHOLARS OF THE COLLEGE OF THE HOLY AND UNDIVIDED TRINITY OF QUEEN ELIZABETH NEAR DUBLIN HEREINAFTER TRINITY COLLEGE DUBLIN

Ireland

THE UNIVERSITY OF MILANO

Italy

UNIVERSIDAD POLITECNICA DE MADRID

Spain

UNIVERSITY OF BRISTOL

United Kingdom

UNIVERSITY OF LEEDS

United Kingdom

UNIVERSITY OF ROME "LA SAPIENZA"

Italy

UPPSALA UNIVERSITY

Sweden

UTRECHT UNIVERSITY

Netherlands

Project information

Grant agreement ID: QLG2-CT-1999-00876

  • Start date

    1 February 2000

  • End date

    1 August 2003

Funded under:

FP5-LIFE QUALITY

  • Overall budget:

    € 11 320 552

  • EU contribution

    € 8 380 162

Coordinated by:

AMICA SCIENCE EEIG

United Kingdom