Cel Bacteria of the genes Rhizobium and Bradyhizobium induce the formation of a new organ, the nodule, on the roots of legumes. During early nodule development, a number of genes (early nodulin genes) are specifically expressed. Two such genesm ENOD5 and ENOD12 from pea are presently being characterized by the Wageningen group. A bacterial factor purified and characterized from Rhizobium meliloti by the Toulouse groups is capable of triggering the early events of nodule formation including the onset of early nodulin gene expression. Several DNA sequences and DNA binding proteins involved in the expression of late nodulin genes have been characterized by the Aarhus group using a legume transformation system. Bringing together the expertise of the three groups we propose a project focusing on a signal transduction pathway of early nodule development, starting from the recognition of the bacterial factor by the plant to the initiation of plant gene expression. In order to investigate the specificity of the bacterial factor, a number of different factors, either chemically modified or products of bacterial mutants, will be tested for their ability to initiate the developmental process leading to nodule formation. This part of the project also provides important knowledge for the identification of plant receptors recogniwing and responding to the bacterial factors. Based on the obtained DNA sequence information on the ENOD5 and ENOD12 genes a series of promoter/reporter gene constructs will be analysed by transient expression assay and in transgenic legumes. The DNA regulatory elements defined by this analysis will be subjected to a protein/DNA binding study, to define trans-acting factors involved in early nodulin expression. The interdepence of the trans-acting factors and the inducing bacterial factors will be analysed. Dziedzina nauki natural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesbiological behavioural sciencesethologybiological interactionsagricultural sciencesagriculture, forestry, and fisheriesagriculturegrains and oilseedslegumes Program(-y) FP2-SCIENCE - Programme plan (EEC) to stimulate the international cooperation and interchange needed by European research scientists (SCIENCE), 1988-1992 Temat(-y) Data not available Zaproszenie do składania wniosków Data not available System finansowania CSC - Cost-sharing contracts Koordynator CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE Wkład UE Brak danych Adres Chemin de Borde-Rouge INRA 31326 AUREVILLE Francja Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (3) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko Centre National de la Recherche Scientifique (CNRS) Francja Wkład UE Brak danych Adres 205 route de Narbonne 31077 Toulouse Zobacz na mapie Koszt całkowity Brak danych Landbouwuniversiteit Wageningen Niderlandy Wkład UE Brak danych Adres 3,Dreijenlaan 6703 HA Wageningen Zobacz na mapie Koszt całkowity Brak danych Århus Universitet Dania Wkład UE Brak danych Adres 10,Gustav Wieds Vej 10 8000 Århus Zobacz na mapie Koszt całkowity Brak danych
