The fight against infectious agents is focused to an efficient treatment of the diseases associated with infections and to the prevention of them. Regarding prevention, the most cost-effective strategy is vaccination. Classical vaccines have used microbe molecules such as cell invasion proteins, critical virulence factors on highly antigenic molecules. However, most of these vaccines have failed to achieve good protection against intracellular bacteria. The most putative reason resides in the ability of microbes to elude the immune response by evolving specific mechanisms to interfere with celldegradative routes (i.e., natural cell defences). Intracellular pathogens could share analogous strategies and compounds for this interference and their characterization will be critical for new and effective vaccines that confer protection against more than one pathogen. On this regard, we plan to design an innovative live, recombinant multi competent vaccine for brucellosis and tuberculosis. Our approach will try to identify and construct a vaccine including the microbe molecules responsible for interfering with cell derivative mechanisms (i.e., the phagosomal transport of lysosomal proteins).