Cel This proposal aims at identifying new biomarkers to improve the current approach for assessing the androgen disruption activity of chemicals (type flu amide) on testicular development and function. The objectives are(I) to use a DNA micro array approach to identify among thousands of testicular genes those whose expression is altered in fatally flu amide treated rats and(II) to delineate the potential role of the proteins encoded by these altered genes in the activities of germ cells, Sirloin and Lading cells. The project is divided in 5 work packages including that concerning the project coordination. The WP2 (DNA micro array preparation) and WP3 (tabular tissues from flu amide treated rats) are performed by the industrial partner. Once the altered genes are identified in WP2 and WP3, experiments based on the in vivo and in vitro expression of these genes and transfixion of these genes in testicular cell lines will delineate their potential role in germ cell (Spa, Sterol cell (WP5) and Lydia cell (WP6) specific activities. From these experiments, we expect to (I) identify key genes involved in testicular path physiology related to an endocrine disruption activity and (ii) provide through these relevant genes new fine biomarkers to assess the ant androgen potential of chemicals. Dziedzina nauki natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesbasic medicinephysiology Program(-y) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Temat(-y) 1.1.1.-4. - Key action Environment and Health Zaproszenie do składania wniosków Data not available System finansowania CSC - Cost-sharing contracts Koordynator INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE Wkład UE Brak danych Adres ADR 5 - Rhône-Alpes, Auvergne, Bourg. Centre Hos 69495 PIERRE-BENITE Francja Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (3) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko BAYER CROPSCIENCE SA Francja Wkład UE Brak danych Adres Rue Dostoievski, 355 06903 SOPHIA ANTIPOLIS Zobacz na mapie Koszt całkowity Brak danych UNIVERSITY OF TURKU Finlandia Wkład UE Brak danych Adres Lemminkaesenkatu, 2 20520 TURKU / ABO Zobacz na mapie Koszt całkowity Brak danych UTRECHT UNIVERSITY Niderlandy Wkład UE Brak danych Adres Sorbonnelaan 16 3584 CA UTRECHT Zobacz na mapie Koszt całkowity Brak danych