Obiettivo A new biotechnological process, exploiting scaffold synthetic repertoires will be developed to profile cancer cells by polemics, interfere with cancer phenotypes, rationally design drugs. The selected scaffold is the SH2 domain, an interaction surface, which binds to phosphotyrosine (pie)-containing peptides. By random autogenesis of five critical residues in the domain we have created library theoretically able to bind every containing-containing protein. This library will be used toa) identify novel specificities of synthetic SH2 domains,b) molecularly dissect the SH2: pY-peptide interaction,c) purify novel pY-proteins,d) interfere with phenotypes such as proliferation, differentiation and apoptosis,e) profile containing patterns in cancer, f) develop peptide antagonists. The involvement of the SH2: pY interaction, in TK-mediated signalling, predicts application of this bioprocess to other pathological conditions, in addition to cancer, involving aproliferative/signaling component. Campo scientifico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesclinical medicineoncology Parole chiave Nanotechnology Programma(i) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Argomento(i) 1.1.1.-3. - Key action The "Cell factory" Invito a presentare proposte Data not available Meccanismo di finanziamento CSC - Cost-sharing contracts Coordinatore ISTITUTO EUROPEO DI ONCOLOGIA SRL Contributo UE Nessun dato Indirizzo Via Ripamonti 435 20141 MILANO Italia Mostra sulla mappa Costo totale Nessun dato Partecipanti (2) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto CANCER RESEARCH TECHNOLOGY LIMITED Regno Unito Contributo UE Nessun dato Indirizzo Sardinia House, Sardinia Street WC2A 3NL LONDON Mostra sulla mappa Costo totale Nessun dato FORSCHUNGSVERBUND BERLIN E.V. Germania Contributo UE Nessun dato Indirizzo 4,Robert-Roessle-Strasse 10 13125 BERLIN Mostra sulla mappa Costo totale Nessun dato
