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Nmr facility for paramagnetic biomolecules

Nmr facility for paramagnetic biomolecules

Objective

Description:
The EU Large Scale Facility PARABIO, established in Florence in the scientific Campus of the University in Sesto Fiorentino, is part of the cluster of Research Infrastructures for Biomolecular NMR. The uniqueness of the infrastructure lies in the widest range of magnetic fields available, which allows a user to combine the two main NMR approaches, namely high resolution NMR and relaxometry. The infrastructure is equipped with an 800 MHz spectrometer, and there are agreements to install a 900 MHz instrument. The infrastructure owns a 200, equipped with CP-MAS accessories, a 400 MHz, a 500 MHz equipped with a CryoProbeÔ, a 600 MHz and two 700 MHz spectrometers. In addition to high field NMR spectrometers, the Florence infrastructure is equipped with several instruments to perform low-field NMR studies. A high-resolution variable field instrument covers the 6-90 MHz field range. A Field Cycling Relaxometer, operating in the 0.01-50 MHz range and a prototype Fast Field Cycling Relaxometer, operating in the 0.001-20 MHz range, are available. With the EC support, PARABIO staff is working at the development of a High Sensitivity Fast Field Cycling NMR (HPRI-CT-2001-50028) that is expected to be available in one year. A parallel computer, a PCs cluster and a large number of networked UNIX and Linux workstations are available for structure determination and refinement and for data analysis in general.

Application:
Main results:
PARABIO gave access to 49 Users from 11 countries, for a total of 952 days spent at the Infrastructure as a result of 76 visits. The spectrometer days delivered were 515, divided in 249 High-Field and 266 days for Low-Field instrumentation. The results of the projects carried out at PARABIO during the present contract were of relevant interest for the scientific community; the resulting works were submitted for publication in peer-reviewed journals and constituted the basis of several Master and Doctoral thesis.

Project Manager:
Ivano Bertini Center for Magnetic Resonance (CERM), Via Luigi Sacconi 6, Sesto Fiorentino (Florence) 50019, Italy
Tel: +39-055-4574272
Fax: +39-055-4574271
E-Mail: bertini@cerm.unifi.it
Leaflet | Map data © OpenStreetMap contributors, Credit: EC-GISCO, © EuroGeographics for the administrative boundaries

Coordinator

CONSORZIO INTERUNIVERSITARIO RISONANZE MAGNETICHE DI METALLOPROTEINE PARAMAGNETICHE

Address

Via L. Sacconi 6 C/O UniversitÀ Di Firenze
50019 Firenze

Italy

Administrative Contact

Ivano BERTINI

Project information

Grant agreement ID: HPRI-CT-1999-00009

  • Start date

    1 January 2000

  • End date

    31 December 2002

Funded under:

FP5-HUMAN POTENTIAL

  • Overall budget:

    € 810 000

  • EU contribution

    € 810 000

Coordinated by:

CONSORZIO INTERUNIVERSITARIO RISONANZE MAGNETICHE DI METALLOPROTEINE PARAMAGNETICHE

Italy