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Feasibility of an eu plant health directive (77/93/eec), diagnostic chip.

Objective

This project will investigate the feasibility of producing a diagnostic chip for the simultaneous detection of all the plant pests/pathogens present in the EU Plant Health Directive (77/93/EEC), using quarantine potato pests as a model system. The project will establish methods for detection of quarantine pathogens in plant tissue using gene chip technology. Up to 30000 DNA probes (sequences from each of the organisms to be tested in one assay) can be arrayed onto a microscope slide - the "gene chip". The chip can be exposed to fluorescent-labelled nucleic acid from the sample and fed into a micro-array reader to reveal if any of the pathogens were present. The project covers the whole process from preparing target genes, generating gene chips, preparing samples, hybridisation of samples, analysis of results and finally through to quarantine diagnosticians evaluating the technology.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

DEPARTMENT FOR ENVIRONMENT, FOOD AND RURAL AFFAIRS
Address
Sand Hutton
YO41 1LZ York
United Kingdom

Participants (4)

DSMZ-DEUTSCHE SAMMLUNG VON MIKROORGANISMEN UND ZELLKULTUREN GMBH
Germany
Address
Mascheroder Weg 1B
38124 Braunschweig
INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
France
Address
Boulevard Francis Meilland 123
06600 Antibes
INSTITUTO VALENCIANO DE INVESTIGACIONES AGRARIAS
Spain
Address
Km 4.5,Ctra. Moncada-naquera Km 4.5
46113 Moncada (Valencia)
UNIVERSITY OF YORK
United Kingdom
Address
Heslington
Y010 5DD York