Objective The objective of the proposal is to produce a detailed description of the molecular mechanisms underlying different stages of virus-induced gene silencing (VIGS) in plant cells. VIGS is a recently discovered sequence - specific RNA degradation mechanism in plants, that has a huge potential for the functional analysis of individual genes on the genome-wide scale, provided that its general principles are better understood. We plan to investigate the following aspects of VIGS: i) viral features detected by the plant^TMs surveillance mechanism; ii) suppressors of VIGS; iii ) host factors required for VIGS; v) role of DNA methylation in VIGS . The fulfilment of the project should pave the path for the construction of generally applicable efficient VIGS vectors for the functional analysis of multitude of plant genes. Fields of science natural sciencesbiological sciencesgeneticsDNAnatural sciencesmathematicspure mathematicsmathematical analysisfunctional analysisnatural sciencesbiological sciencesgeneticsRNA Programme(s) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Topic(s) 1.1.1.-8. - Research into genomes and diseases of genetic origin Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator NATIONAL INSTITUTE OF CHEMICAL PHYSICS AND BIOPHYSICS EU contribution No data Address 23,Akademia Tee 23 12618 TALLINN Estonia See on map Total cost No data Participants (7) Sort alphabetically Sort by EU Contribution Expand all Collapse all AGRICULTURAL BIOTECHNOLOGY CENTRE Hungary EU contribution No data Address Szent Gyorgyi A 4 2100 GODOLLO See on map Total cost No data CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS Spain EU contribution No data Address Campus de la Universidad Autonoma de Madrid 28049 MADRID See on map Total cost No data FOUNDATION OF RESEARCH AND TECHNOLOGY - HELLAS Greece EU contribution No data Address Vassilika Vouton 71110 IRAKLION See on map Total cost No data KEYGENE NV Netherlands EU contribution No data Address 90, 90 Agro business Park 6700 AE WAGENINGEN See on map Total cost No data THE PROVOST, FELLOWS AND SCHOLARS OF THE COLLEGE OF THE HOLY AND UNDIVIDED TRINITY OF QUEEN ELIZABETH NEAR DUBLIN HEREINAFTER TRINITY COLLEGE DUBLIN Ireland EU contribution No data Address Smurfit Institute,Trinity College 2 DUBLIN See on map Total cost No data THE SAINSBURY LABORATORY United Kingdom EU contribution No data Address Norwich Research Park, Colney Lane NR4 7UH NORWICH See on map Total cost No data WAGENINGEN UNIVERSITY Netherlands EU contribution No data Address Binnenhaven, 11 6709 PD WAGENINGEN See on map Total cost No data
