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Ciliates as monitors for environmental safety of gmo

Project information

Grant agreement ID: QLK3-CT-2002-02151

  • Start date

    1 October 2002

  • End date

    31 December 2005

Funded under:

FP5-LIFE QUALITY

  • Overall budget:

    € 2 226 400

  • EU contribution

    € 1 848 917

Coordinated by:

STICHTING KATHOLIEKE UNIVERSITEIT

Netherlands

Objective

One of the major concerns limiting the potential usage of genetically modified crops and organisms in agriculture is the potential risk for lateral gene transfer in the environment. While numerous studies have considered lateral gene transfer into gut bacteria, the transfer into unicellular eukaroytes has been ignored until now. Here we propose that the unique genome organisation of ciliated protozoa, i.e. the presence of an asexual macronucleus with gene-sized mini-chromosomes, makes them ideal bio-monitors for gene transfer events in the gut of farm animals and insects. The telomeric sequences of gut ciliates plus signature sequences in GMO-derived DNA should allow primers to be developed for bulk DNA analysis by PCR and high throughput screening using fluorescence in situ hybridisation and flow cytometry. These transfer events will be compared to historical gene transfer event evaluated by bioinformatical techniques.

Coordinator

STICHTING KATHOLIEKE UNIVERSITEIT

Address

Netherlands

Participants (4)

INSTITUTE OF ANIMAL PHYSIOLOGY - SLOVAK ACADEMY OF SCIENCES

Slovakia

ROWETT RESEARCH INSTITUTE

United Kingdom

TECHNICAL UNIVERSITY OF MUNICH

Germany

THE KIELANOWSKI INSTITUTE OF ANIMAL PHYSIOLOGY AND NUTRITION - POLISH ACADEMY OF SCIENCES

Poland

Project information

Grant agreement ID: QLK3-CT-2002-02151

  • Start date

    1 October 2002

  • End date

    31 December 2005

Funded under:

FP5-LIFE QUALITY

  • Overall budget:

    € 2 226 400

  • EU contribution

    € 1 848 917

Coordinated by:

STICHTING KATHOLIEKE UNIVERSITEIT

Netherlands