Objetivo Listeria monocytogenes emerged in the mid-eighties as a major food borne infectious agent, and up-to-date it poses a major public health issue in view of high mortality rate (up to 30%) in the declared incidence. The given project has its ultimate goal to yield improved options for risk management of Listeria monocytogenes along the food chain, and to identify the conditions needed to minimise its survival and growth.The research program of the project includes the following items:Elaboration of practically methods of express-diagnostics and typing of Listeria monocytogenes strains, which allow to control the pathogen along all food chain, from a farm to a consumer;Investigation of Listeria monocytogenes survival, growth and virulence in environmental models, simulating natural habitants like soil, food and animal tissues;Comparative epidemiological analysis of clinical and food borne isolates all around the Europe to get insight into the relationships between epidemiological, physiological and virulent characteristics of this species;Study of genetic and physiologic bases of listerial survival, growth and virulence expression under specific stress conditions observed in food models.The project aims to perform the following Tasks :To compare and evaluate the practical importance of PCR (Polymerase Chain Reaction)-based techniques for simultaneous identification and typing of Listeria monocytogenes directly in food products;To elaborate reagents and protocols for serologic monitoring of Listeria monocytogenes in herds and in food;To develop quantitative models for evaluation of Listeria monocytogenes potential to survive in native environments;To understand mechanisms of the prevalence of certain genotypes in defined conditions;To characterise the transcriptional and post-transcriptional regulatory pathways and networks involved in survival in a model food products;To characterise the role of central virulence regulator PrfA in adaptive response;To identify genes selectively induce during the adaptive response of Listeria monocytogenes in food products.The main objective of the project is the decrease of the risk of food product contamination with the pathogen Listeria monocytogenes to avoid outbreaks as well as sporadic cases of listeriosis. Programa(s) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Tema(s) 4 - Life Sciences FOOD - Food quality and safety Convocatoria de propuestas Data not available Régimen de financiación Data not available Coordinador Complutense University of Madrid Aportación de la UE Sin datos Dirección Avd. Puerta de Hierro s/n 28040 Madrid España Ver en el mapa Coste total Sin datos Participantes (4) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo All-Russia Meat Institute Rusia Aportación de la UE Sin datos Dirección Talalikhin str.26 109316 Moscow Ver en el mapa Coste total Sin datos Gamaleya Research Institute of Epidemiology and Microbiology Rusia Aportación de la UE Sin datos Dirección Gamaleya st.18 123098 Moscow Ver en el mapa Coste total Sin datos Research Institute of veterinary virology and microbiology Rusia Aportación de la UE Sin datos Dirección 601120 Pokrov Ver en el mapa Coste total Sin datos University of Veterinary Medicine Austria Aportación de la UE Sin datos Dirección Veterinarplatz 1 1210 Wien Ver en el mapa Coste total Sin datos
