Objectif The endoplasmic reticulum (ER) calcium stores are an indispensable source for Ca2+ signals involved in physiological responses of various types of non-excitable and excitable cells. The ER membrane in essence forms an intracellular excitable media, which can produce both synchronous release and calcium waves within single cells and multicellular networks. The objective of this project is to test the hypothesis that the local Ca2+ release from the ER store present in the synaptic terminal is involved in the regulation of neurotransmitter release and synaptic plasticity. This hypothesis, though being proposed previously, has not been proven yet by direct experimental observations.The experiments will be performed on a low-density primary culture of rat hippocampal neurones. Presynaptic [Ca2+]i and postsynaptic membrane currents will be measured simultaneously by combination of whole-cell patch-clamp recordings and fluorescent video-imaging. The single synaptic terminals will be visualised by FM1-43 staining; resulting image will be used as a region of interest for [Ca2+]i recordings.The experiments will be designed to(i) visualise ER Ca2+ store in the single synaptic terminal;(ii) investigate [Ca2+]i dynamics in a single synaptic terminal in response to stimulation of Ca2+ release from the ER;(iii) correlate presynaptic [Ca2+]i dynamics with synaptic strength, measured by postsynaptic currents monitoring;(iv) identify the involvement of ER Ca2+ store in regulation of synaptic transmission at the level of single terminal and;(v) develop a mathematical model describing the relations between Ca2+ entry/Ca2+ release during presynaptic excitation and the relative importance of these pathways in regulating synaptic plasticity. Programme(s) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Thème(s) 4 - Life Sciences OPEN - OPEN Call Appel à propositions Data not available Régime de financement Data not available Coordinateur University of Manchester Contribution de l’UE Aucune donnée Adresse Oxford Road M13 9PT Manchester Royaume-Uni Voir sur la carte Coût total Aucune donnée Participants (3) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire Dniepropetrovsk National University Ukraine Contribution de l’UE Aucune donnée Adresse Naukovy provulok 13 49050 Dniepropetrovsk Voir sur la carte Coût total Aucune donnée Faculty of Pharmacy Italie Contribution de l’UE Aucune donnée Adresse Corso Raffaello 30 10125 Turin Voir sur la carte Coût total Aucune donnée National Academy of Sciences of Ukraine Ukraine Contribution de l’UE Aucune donnée Adresse Bogomoletz str., 4 252024 Kiev Voir sur la carte Coût total Aucune donnée
