Obiettivo The project is designed to investigate the role of specific enzymes, namely metalloproteases, especially belonging to the ADAMs (a disintegrin and metalloprotease) family, in the development of the brain, learning and memory under normal conditions and after severe hypoxia, which is one of the most common complications accompanying impaired brain metabolism caused by various internal and environmental factors. The hypothesis is that there may be various types of correlation between the levels of expression of different metalloproteases of the ADAMs and NEP families in the brain structures participating in the control of motor behaviour, learning and memory (sensorimotor cortex, striatum, amygdala and, hippocampus) of the brain at different stages of postnatal development that will be significantly influenced by hypoxia with and without preconditioning. To this end the analysis of expression of several metalloproteases (ADAMs 9, 10, 15 and 17) and DINE (damage induced neuronal endopeptidase) in brain structures regulating learning and memory at different stages of development under normal conditions and after severe hypoxia with and without preconditioning will be performed.This complex study of the problem at molecular, morphological and behavioural levels will reveal a correlation between the expression of different metalloproteases in various brain structures with the level of motor behaviour and learning abilities of animals before and after severe hypoxia. The specific characteristics of ADAM expression in vulnerable (CA1/CA2 of hippocampus, striatum, neocortex) and tolerant (dentate gyrus, thalamus) regions will also be explored. Administration of specific inhibitors of metalloproteases into the structures controlling motor behaviour and formation of new reflexes with analysis of short-term memory of rats will testify exactly which metalloproteases might be involved in the process of learning and memory. These data will be further interpreted from the point of view of their possible implication for the development of a therapeutic strategy for the treatment of impaired brain functions caused by ischemic insults and neurodegenerative diseases. Programma(i) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Argomento(i) 4 - Life Sciences OPEN - OPEN Call Invito a presentare proposte Data not available Meccanismo di finanziamento Data not available Coordinatore University of Leeds Contributo UE Nessun dato Indirizzo Mount Preston Street LS2 9JT Leeds Regno Unito Mostra sulla mappa Costo totale Nessun dato Partecipanti (3) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto Russian Academy of Sciences Russia Contributo UE Nessun dato Indirizzo M. Thorez Prospect, 44 194223 St. Petersburg Mostra sulla mappa Costo totale Nessun dato Russian Academy of Sciences Russia Contributo UE Nessun dato Indirizzo 2 Bykobva Street 188680 Koltushi, Leningrad Region Mostra sulla mappa Costo totale Nessun dato University of Tampere Finlandia Contributo UE Nessun dato Indirizzo 6 Lenkkeilijankatu 33520 Tampere Mostra sulla mappa Costo totale Nessun dato
