Obiettivo Participation of simultaneously nitrosylated and farnesylated Ras-protein in neuronal apoptosis during brain ischaemia and experimental allergic encephalomyelitis.Ras proteins are proto-oncogene products that are critical components of signalling pathways leading from cell-surface receptors to the control of cellular proliferation, differentiation or cell death. Activation of Ras is associated with ischaemia/reperfusion damage of brain or with neuroinflammatory diseases. Post-translational modification is responsible for the proper functioning of Ras. The main scientific objective of the project is the investigation of role of nitrosylation on the membrane-binding properties of Ras and downstream effectors specificity of simultaneously (farnesylated and nitrosylated) modified isoforms of Ras.The project is directed towards the following points:(a) Purification, isolation, preparation, and investigation of biological activities of double modified isoforms (simultaneously nitrosylated and farnesylated) of Ras;(b) Investigation of adhesive and phospholipid-binding properties of various modified isoforms of Ras using a highly sensitive integrated optical technique allowed detailed and sophisticated analysis of the entire binding phenomenology;(c) Investigation of the action of farnesyltransferase and nitric oxide synthase inhibitors on the induction/prevention of neuronal damage during brain ischaemia and experimental allergic encephalomyelitis in rats.The anticipated results will reveal the functionally active form of Ras which participates in different downstream processes of the cell. After successful completion of the project, it will be possible to recommend types of pharmacological drugs, which will diminish the apoptosis/necrosis of neuronal cells after ischaemia/reperfusion damage of brain or after neuroinflammation. Programma(i) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Argomento(i) 4 - Life Sciences OPEN - OPEN Call Invito a presentare proposte Data not available Meccanismo di finanziamento Data not available Coordinatore Collegium Basilea Contributo UE Nessun dato Indirizzo Hochstrasse 51 4053 Basel Svizzera Mostra sulla mappa Costo totale Nessun dato Partecipanti (4) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto Georgian Academy of Sciences Georgia Contributo UE Nessun dato Indirizzo Gotua St 14 380060 Tbilisi Mostra sulla mappa Costo totale Nessun dato Ministry of Health Armenia Contributo UE Nessun dato Indirizzo Orbeli St. 41 375028 Erevan Mostra sulla mappa Costo totale Nessun dato State Medical Academy of Postgraduate Education Georgia Contributo UE Nessun dato Indirizzo Chavchavadze Ave. 29 380079 Tbilisi Mostra sulla mappa Costo totale Nessun dato University of P?cs Ungheria Contributo UE Nessun dato Indirizzo Szigeti 12 7624 Pécs Mostra sulla mappa Costo totale Nessun dato