Objectif The programme bears on the development of enzyme electrodes using the hydrogenase enzyme to transfer electrons between the electrode and molecular hydrogen. The ultimate goal is the clean production of energy, in the form of either H2 or electricity, by combining electrochemistry with enzymatic reactions. A major objective will be the development of inexpensive and efficient hydrogenase electrodes for H2 production.For this purpose, the strategies of biomolecular immobilization developed at Grenoble (team of Dr S. Cosnier) and Moscow (team of Dr A. Karyakin) will be compared in terms of retention of enzymatic activities and efficiency of the electrical wiring step. A strong synergy is expected through the complementary interactions between the Grenoble and Moscow teams. In particular, the association of the capability in the field of biosensor construction (Grenoble) with the competence in the field of bioelectrocatalysis and electrode surface treatment (Moscow) should lead to the development of powerful configurations of enzyme electrodes.Three biological laboratories participate to the project. The three laboratories currently study the hydrogenase enzyme and H2 metabolism in phototrophs. They are used to exchange informations between them and to collaborate. The task of the biochemists and microbiologists of Pushchino (team of Dr N. A. Zorin) will be to furnish the hydrogenase enzyme to be immobilized onto the electrode and to select from natural strains the best hydrogenase proteins (with respect to stability towards O2, pH, temperature, and with high activity). New purification procedures will be tested to increase the yield of enzyme and the speed of the purification step. The molecular biologists (team of Dr P.M. Vignais, Grenoble and Dr P. Lindblad, Uppsala) will contribute to the identification of new hydrogenase genes in the photosynthetic bacteria and the cyanobacteria. They will construct deregulated mutant strains overproducing active hydrogenase, which will be grown in Pushchino in continuous cultures. Besides, in tight collaboration with the electrochemists, the molecular biologists will design new forms of hydrogenase protein so as to overcome the constraints due to the fabrication of an enzyme electrode. Thus, the functionalization of hydrogenase proteins will contribute to improve the electrical communication or to develop new attractive immobilization procedures.Given the competence of the five participating groups, a competence which goes from the extraction of the hydrogenase protein to the electrical connection of the enzyme, an extremely fruitful symbiotic and synergistic collaboration is expected. Programme(s) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Thème(s) 3 - Chemistry INTAS - INTAS Appel à propositions Data not available Régime de financement Data not available Coordinateur Université Joseph Fourier Grenoble Contribution de l’UE Aucune donnée Adresse Rue de la Chimie 301 38041 Grenoble Cedex 9 France Voir sur la carte Coût total Aucune donnée Participants (4) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire CEA Grenoble France Contribution de l’UE Aucune donnée Adresse 17 Avenue des Martyrs 38054 Grenoble Cedex 9 Voir sur la carte Coût total Aucune donnée Institute of Soil Science and Photosynthesis Russie Contribution de l’UE Aucune donnée Adresse pr. Nauki 142292 Pushchino, Moscow Region Voir sur la carte Coût total Aucune donnée M.V. Lomonosov Moscow State University Russie Contribution de l’UE Aucune donnée Adresse Vorobjevy Gory 119899 Moscow Voir sur la carte Coût total Aucune donnée University of Uppsala Suède Contribution de l’UE Aucune donnée Adresse Villavagen 6 752 36 Uppsala Voir sur la carte Coût total Aucune donnée
