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Interaction between components of the cytoskeleton and Marburg and Ebola virus nucleocapsids in infected cells

Objective

Marburg and Ebola virus make up the family of Filoviridae, which are among the most dangerous viral pathogens. Filoviruses reproduce in the cytoplasm of the infected cell where prominent inclusion bodies are formed which represent stored nucleocapsids. Nucleocapsids undergo several steps of maturation and have then to be transported to the plasma membrane where they are enveloped, and progeny viruses are released into the cultural medium. Hitherto the mechanisms underlying the transport of mature nucleocapsids to the plasma membrane are not understood. Additionally, it is unclear how mature and premature nucleocapsids are discriminated. It is presumed that the cytoskeleton of the infected cells plays an important role in these processes. The proposed project wants to elucidate the importance of acting filaments and microtubules for the transport of filoviral nucleocapsids. This study combines electron microscopical, immuno electron microscopical, molecular biological, and light microscopical analyses to get a detailed picture of nucleocapsid migration and virus morphogenesis. It is expected that gained results lead to a better understanding of the intimate relationship between cellular structures and the viral parasite. Further, these investigations will provide the fundament to develop drugs blocking viral morphogenesis.

Coordinator

Philipps University Marburg
Address
Robert-koch-str. 17
35037 Marburg
Germany

Participants (3)

Erasmus University of Rotterdam
Netherlands
Address
Dr Molewaterplein 50
3015 Rotterdam
Novosibirsk State University
Russia
Address
Pirogova 1
630090 Novosibirsk
State Research Center of Virology and Biotechnology
Russia
Address
Koltsovo
633159 Novosibirsk Region