Objectif The initial stage of organic toxicants degradation in plants is their oxidative transformation. As it is well known, this is rate limiting in multi-step complicated process of detoxification. According to different considerations xenobiotic oxidation in plants is carried out via monooxygenase, or via peroxidase, or via phenoloxidase. On the basis of our investigations, we can suppose that none of the stated mechanism is not universal way for xenobiotic detoxification. Consequently, the hypothesis is drawn out, according to which, the interchange of oxidative systems in xenobiotic detoxification takes place, which should be determined by many internal and external factors.The importance of this research is to determine exactly what enzymatic systems participate in the first step of xenobiotics oxidation - hydroxylation and to establish the mechanism of their coordination.The goal of the research is the establishment of possible pathways of nitrobenzene oxidation in soybean and maize plants; the reveal of the fact of different oxidative enzyme systems interchange in xenobiotic hydroxylation.To achieve the Goal following tasks will be addressed:Effect of xenobiotic on cell ultrastructure.Xenobiotic oxidation by monooxygenase, peroxidase and phenoloxidase enzymatic systems in subcellular fractions.Quantitative input of each enzymatic mechanisms in xenobiotic oxidation.The intensity of oxidation processes in relation with plant cultivation regime and age. Variants: etiolated plant seedlings; seedlings grown in light; seedlings infected with pathogenic fungus; seedlings grown on xenobiotic containing solutions; different age seedlings.The expected results will contribute for more clear understanding of the mechanism of interchange, whether it is realized: by different oxidation enzymes interchange each other; by the modification of cytrochrome P450 activity; or by the combination of the two mechanisms. Programme(s) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Thème(s) 4 - Life Sciences GEORGIA - GEORGIA Appel à propositions Data not available Régime de financement Data not available Coordinateur INRA Contribution de l’UE Aucune donnée Adresse Chemin de Tournefeuille, 180 31931 Toulouse Cedex 9 France Voir sur la carte Coût total Aucune donnée Participants (3) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire Durmishidze Institute of Plant Biochemistry Géorgie Contribution de l’UE Aucune donnée Adresse David Agmasheneblis Kheivani 10km 380059 Tbilisi Voir sur la carte Coût total Aucune donnée Georgian Agrarian University Géorgie Contribution de l’UE Aucune donnée Adresse David Agmasheneblis Kheivani 13km 380059 Tbilisi Voir sur la carte Coût total Aucune donnée Technische Universitat Munchen Allemagne Contribution de l’UE Aucune donnée Adresse Schulstrasse, 10 85356 Freising Voir sur la carte Coût total Aucune donnée
