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Biosensors for in situ evaluation of bioavailability of pollutants based on transcriptional regulators a la carte.

Project information

Grant agreement ID: QLK3-CT-2002-01923

  • Start date

    1 November 2002

  • End date

    30 April 2006

Funded under:

FP5-LIFE QUALITY

  • Overall budget:

    € 1 963 013

  • EU contribution

    € 1 025 764

Coordinated by:

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

Spain

Objective

This Project exploits the properties of prokaryotic transcriptional regulators of the XylR/HbpR family to design a la carte regulators that will be employed to determine the bioavailability of nitro-, broom- and chloroaromatic chemicals and biphenyl ethers. The core of this proposal is the application of high-throughput combinatory genetic technologies to the protein module that sets the specificity of the response to distinct aromatics. This will allow the generation and selection of regulator variants able to recognize and to trigger responses to a large variety of chemical species. The selected proteins will be employed in various biosensor schemes through the coupling of the cognate promoters to genetically formatted lox and gap reporters. This genetic standardisation will going parallel with matching instrumentation, hardware and software suited for measuring the optical output of the system and its utilisation in the field.

Coordinator

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

Address

1,Profesor Albareda 1
18008 Granada

Spain

Participants (4)

BIOSENSORES SL.

Spain

THE SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES

Sweden

UNIVERSITY OF ABERDEEN

United Kingdom

UNIVERSITY OF LAUSANE

Switzerland

Project information

Grant agreement ID: QLK3-CT-2002-01923

  • Start date

    1 November 2002

  • End date

    30 April 2006

Funded under:

FP5-LIFE QUALITY

  • Overall budget:

    € 1 963 013

  • EU contribution

    € 1 025 764

Coordinated by:

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

Spain