The aim of BIODIOL is to develop an integrated biotechnical process for the production of the bulk-chemical l,3-propanediol (PD) from glycerol waters by using directly the biological waste from biodiesel plants. To increase PD yield use of wastes from food industries as co-substrate will be investigated. To enable the usage of these cheap impure substrates necessary desalting will be done by electrodialysis. A new, highly product-tolerant strain will be used in immobilised form for enhanced productivity and process stability. To gain high PD concentrations, a (fed) -batch system will be set-up. For process control an online -sensor will be developed to monitor the concentration of the substrates. To prevent bacteria inhibition and facilitate PD-recovery, organic acids as by- products will be removed by electrodialysis. All work will be accompanied by economical rating of the individual steps.