Skip to main content

Radiation specific DNA non-double strand break lesions: repair mechanisms and biological effects

Objective

Ionising radiation induces a plethora of DNA non-double strand break lesions such as oxidised bases, a basic sites and single strand breaks. Strong evidence exists that ionising radiation induces, besides isolated DNA lesions; a unique form o f DNA damage termed clustered damage consisting of closely spaced lesions on opposite DNA strands. There is also evidence that repair of lesions within clustered damage sites is compromised compared with the effective repair of isolated lesions.

We will investigate the following aspects of radiation-induced single and clustered DNA damage: (i) frequency and nature of the damage, (ii) mechanisms of repair, (iii) interference with transcription, replication and chromatin dynamics and (iv) biological consequences (stress responses, mutations, lethality). To achieve these goals we will exploit a variety of new technologies and utilise different organisms with defined mutations in damage response genes.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

LEIDEN UNIVERSITY
Address
Wassenaarseweg 72
2300 RA Leiden
Netherlands

Participants (6)

COMMISSARIAT A L'ENERGIE ATOMIQUE
France
Address
Avenue Du General Leclerc 60-68
92265 Fontenay Aux Roses
ERASMUS UNIVERSITEIT ROTTERDAM
Netherlands
Address
Dr. Molewaterplein 50
3000 DR Rotterdam
GSF-RESEARCH CENTER FOR ENVIRONMENT AND HEALTH
Germany
Address
Ingolstaedter Landstrasse 1
85764 Oberschleissheim
INSTITUT CURIE
France
Address
Rue D'ulm 26
75248 Paris
MEDICAL RESEARCH COUNCIL
United Kingdom
Address
Harwell
OX11 0RD Didcot
UNIVERSITY OF SUSSEX
United Kingdom
Address
Falmer
BN1 9RR Brighton / Falmer