Ionising radiation induces a plethora of DNA non-double strand break lesions such as oxidised bases, a basic sites and single strand breaks. Strong evidence exists that ionising radiation induces, besides isolated DNA lesions; a unique form o f DNA damage termed clustered damage consisting of closely spaced lesions on opposite DNA strands. There is also evidence that repair of lesions within clustered damage sites is compromised compared with the effective repair of isolated lesions.
We will investigate the following aspects of radiation-induced single and clustered DNA damage: (i) frequency and nature of the damage, (ii) mechanisms of repair, (iii) interference with transcription, replication and chromatin dynamics and (iv) biological consequences (stress responses, mutations, lethality). To achieve these goals we will exploit a variety of new technologies and utilise different organisms with defined mutations in damage response genes.
Funding SchemeCSC - Cost-sharing contracts
92265 Fontenay Aux Roses
3000 DR Rotterdam
OX11 0RD Didcot
BN1 9RR Brighton / Falmer