Obiettivo The turnover and remodelling of extra cellular matrix (ECM) is an important feature after vascular injury in noontime formation and remodelling of the injured vessels. Collagen is the major ECM protein and can only be degraded by the matrix metalloproteases (Mumps). Broad spectrum MMP-inhibitors can prevent arterial shrinkage and noontime formation but suffer from severe side effects after prolonged administration in humans. To find a specific target(s) for intervention, we have to understand how turnover of collagen is regulated. Collagen degradation is dependent on the action of several matrix metalloproteinases and MMP activity is regulated at multiple levels. The expression ofMT1-MMP, MMP2 and MMP-9 are early unregulated in time after vascular injury. Although, the activation of MT-1MMP(MMP2) takes place on the cell surface the compartmental basis for integrating the Tran membrane signalling underlying the MMP2 activation cascade into cells remains to be established. MMP2, MT1-MMP and TIMP-2 are co-localized with Caveolin-1, a major constituent of theCaveolae. The cellular functions of Cavalla are not yet fully understood. Recently, the study of sub cellular distribution of carboxyl-terminally green fluorescent protein GFP-tagged Cav-1 (Cav-1-GFP) revealed that this protein has high mobility within cells. Cav-1 can be found at the cell surface, associated with Googly complex and present in alveolar vesicles. Cav-1,moves bi-directional between the cell surface and cell interior. Several reports have shown that certain viruses, lipid-bound toxins and certain glycosylphosphosphatidylinositol (GPI)- linked proteins enter cells and are sorted to different sub cellular locations bypassing endoscopes using a recently described organelle (termed the \"Cave some\"). This organelle has been visualized by electronic microscopy and it lacks markers for early-endoscopes, liposomes, ER or Googly complex but are rich in caveolin-1. Campo scientifico natural sciencesbiological sciencesmicrobiologyvirologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesphysical sciencesopticsmicroscopy Parole chiave 1 Caveosome RNA interference collagen turnover Programma(i) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Argomento(i) MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF) Invito a presentare proposte FP6-2002-MOBILITY-5 Vedi altri progetti per questo bando Meccanismo di finanziamento EIF - Marie Curie actions-Intra-European Fellowships Coordinatore UNIVERSITY MEDICAL CENTER UTRECHT. HEART LUNG CENTER UTRECHT Contributo UE Nessun dato Indirizzo Heidelberglaan 100, Room G02-523 UTRECHT Paesi Bassi Mostra sulla mappa Costo totale Nessun dato
