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DIETARY EXPOSURES TO POLYCYCLIC AROMATIC HYDROCARBONS AND DNA DAMAGE

Objective

DNA damages that are not repaired during cell proliferation represent initiating events that may result in neoplasia. Exposure from polycyclic aromatic hydrocarbons (PAHs) from some foods is sufficiently high to permit direct studies of such DNA lesions in humans. This potential health hazard - its modulation by genetic factors, synergists and protective substances - constitutes the main focus for this proposal. The intent is also to provide new methods for assessing oxidative and other types of DNA damage as well as basic knowledge on different DNA repair mechanisms. The impact from exposures via food to PAHs will be studied in women characterized by different ethnicity and life styles from the general population in Poland, Serbia and Italy, as well as in subjects living in two environmental catastrophe zones in Serbia. Arsenic enhances the genotoxic effects of PAHs in model systems, and the proposed investigation of the effects of combined exposures to PAHs and high levels of arsenic in drinking water is unique, as is the assessment of individual DNA repair capacity, which offers a possibility to identify particularly sensitive groups with respect to this type of carcinogens. In humans the utility of various biomarkers derived from model systems, and that are relevant for tumour induction by PAHs, will be directly assessed by monitoring chromosomal and DNA damage in buccal cells and lymphocytes. The comparative studies to be conducted in the mouse on inhibition of PAH-induced genotoxicity by selected plant constituents provide an input to a subsequent investigation in humans. In this study the inhibiting effects of anticarcinogens with respect to DNA damage and chromosomal aberrations induced by exposure to PAHs will be assessed directly in isolated lymphocytes and buccal cells from human volunteers in an intervention cross-over study. In the area of public health, efforts will be made to promote a healthier diet for the general population.

Call for proposal

FP6-2002-FOOD-1
See other projects for this call

Funding Scheme

STREP - Specific Targeted Research Project

Coordinator

NOFER INSTITUTE OF OCCUPATIONAL MEDICINE
Address
Teresy Street 8
199 Lodz
Poland

Participants (7)

STOCKHOLM UNIVERSITET
Sweden
Address
Universitetsvaegen 10
Stockholm
KAROLINSKA INSTITUTET - KI
Sweden
Address
Solnavägen 1
Stockholm
INSTITUTE OF EXPERIMENTAL MEDICINE - ACADEMY OF SCIENCES OF THE CZECH REPUBLIC
Czechia
Address
Videnska 1083
Praha 4
UNIVERSITA DEGLI STUDI DELLA TUSCIA
Italy
Address
Via San Giovanni Decollato 1
Viterbo
KLINICKI CENTAR "DR. SIMA MILOSEVIC"
Address
Pozeska 82
Belgrade
BIOCHEMICAL INSTITUTE FOR ENVIRONMENTAL CARCINOGENS - PROF. DR. GERNOT GRIMMER FOUNDATION
Germany
Address
Lurup 4
Grosshansdorf
INSTITUT ZA NUKLEARNE NAUKE VINCAS
Address
Mike Petrovica Alasa Bb
522 Vinca-belgrade