Obiettivo Introduciton: Unlike monoclonal antibodies from hybridomas, recombinant antibodies from bacteria usually suffer from poor stability and yield. Due to surface displayed antibody molecules, recombinant antibodies are easily screened, though. (WP1) In order to combine both advantages, we plan to construct a library of >10(+6) different hybridomas by consecutive homologous and specific recombinations, with all of the hybridomas descending from one single cell. Each dell displays >100.000 of "its" specifi c antibody molecules on the cell surface. Therefore, the library should be easily screened fo low to medium affinity human antibodies specific for almost all antigens. (WP2) Antibodies of higher affinities will be generated by the apparatus responsible fo r somatic hypermutations currently being deciphered, whereby FACS-screening offers an especially easy method to compare affinities. (WP3) The ancestor cell line of our antibody library is engineered to emit signals in response to antigen binding, thus a llowing for the labelling detection of antigen-specific antibodies. Outlook: The combined efforts described above might evolve into a method that supersedes the current state of the art in the production of monoclonal antibodies. Campo scientifico natural sciencesbiological sciencesmicrobiologybacteriology Programma(i) FP6-POLICIES - Policy support: Specific activities covering wider field of research under the Focusing and Integrating Community Research programme 2002-2006. Argomento(i) NEST-2003-1 - Adventure activities Invito a presentare proposte FP6-2003-NEST-PATH Vedi altri progetti per questo bando Meccanismo di finanziamento STREP - Specific Targeted Research Project Coordinatore DEUTSCHES KREBSFORSCHUNGSZENTRUM HEIDELBERG Contributo UE Nessun dato Indirizzo Im Neuenheimer Feld 280 HEIDELBERG Germania Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato Partecipanti (4) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto BEREICH HUMANMEDIZIN DER GEORG-AUGUST-UNIVERSITAET - STIFTUNG DES OEFFENTLICHEN RECHTS Germania Contributo UE Nessun dato Indirizzo Robert-Koch-Str. 40 GOETTINGEN Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato EUCODIS GMBH Austria Contributo UE Nessun dato Indirizzo Brunner Strasse 59 VIENNA Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE Francia Contributo UE Nessun dato Indirizzo 101 Rue de Tolbiac PARIS Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato INSTITUTE OF MOLECULAR GENETICS, ACADEMY OF SCIENCES OF THE CZECH REPUBLIC Cechia Contributo UE Nessun dato Indirizzo Flemingovo namesti 2 PRAGUE 6 Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato