Objetivo Chirality is a key factor in the efficacy of many drugs and the production of single enantiomers of chiral intermediates has therefore become increasingly important. Biocatalysis offers high enantioselectivity and regioselectivity in chiral synthesis through enzyme-catalyzed reactions and thus has an important advantage over chemical synthesis. Molecular genomic data is an unprecedented resource of enzymes for biocatalysis, but rational and effective methodologies must be established to realize the full potential of these resources. This project will focus on the discovery of novel enzymes, from both public and proprietary eubacterial genomes, in particular novel alcohol dehydrogenases, cytochrome P450 monooxygenases and amino acid modifying enzymes for use in established and innovative processes for chiral synthesis.The DATAGENOM project extends from genome analysis, through cloning, expression, enzyme production, screening and protein engineering, to the enzymatic production of chiral biomolecules. The design of the project takes advantage of broad funnel-approach starting with innovative data-mining and processing of a large number of genes to ensure high flow- through in the process and rational selection of best enzyme candidates. The specific combination of expertise and design of the research project is aimed at high success-rate for the development of successful biocatalysts. Emphasis will be put on effective bioinformatics analysis to minimize the requirement for the more laborious "wet chemistry" analysis as well as development of optimised vector-host systems for efficient gene expression and enzyme production. Rational protein engineering or directed molecular evolution will be employed in order to obtain more robust variants, new substrate preferences or enhanced enantiomeric selectivity. Ámbito científico natural sciencesbiological sciencesgeneticsDNAnatural scienceschemical sciencescatalysisbiocatalysisnatural scienceschemical sciencesorganic chemistryaminesnatural sciencesbiological sciencesgeneticsgenomesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Palabras clave Genomes chiral biomolecules enzymes metagenome Programa(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Tema(s) LSH-2002-1.2.5-4 - Exploiting post genomics to produce optically active therapeutic biomolecules by biocatalysis Convocatoria de propuestas FP6-2002-LIFESCIHEALTH Consulte otros proyectos de esta convocatoria Régimen de financiación STREP - Specific Targeted Research Project Coordinador PROKARIA EHF Aportación de la UE Sin datos Dirección Gylfaflot 5 REYKJAVIK Islandia Ver en el mapa Coste total Sin datos Participantes (8) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo INGENZA LTD. Reino Unido Aportación de la UE Sin datos Dirección Caslte street, 39 EDINBURGH Ver en el mapa Coste total Sin datos LUNDS UNIVERSITET Suecia Aportación de la UE Sin datos Dirección Paradisgatan 5c LUND Ver en el mapa Coste total Sin datos UNIVERSITAET STUTTGART Alemania Aportación de la UE Sin datos Dirección Keplerstrasse 7 STUTTGART Ver en el mapa Coste total Sin datos UNIVERSIDAD DE OVIEDO España Aportación de la UE Sin datos Dirección Plaza de Riego no 4 OVIEDO Ver en el mapa Coste total Sin datos DEGUSSA AG Alemania Aportación de la UE Sin datos Dirección Benningsenplatz 1 HANAU-WOLFGANG Ver en el mapa Coste total Sin datos BIOINFOBANK INSTITUTE Polonia Aportación de la UE Sin datos Dirección Limanowskiego 24/a POZNAN Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos JFC - JUELICH FINE CHEMICALS GMBH Alemania Aportación de la UE Sin datos Dirección Rudolf-Schulten-Strasse 5 JUELICH Ver en el mapa Coste total Sin datos CHRISTIAN-ALBRECHTS-UNIVERSITAET ZU KIEL Alemania Aportación de la UE Sin datos Dirección OLSHAUSENSTRASSE 40 KIEL Ver en el mapa Coste total Sin datos