Cel There is an urgent clinical requirement for appropriate bone substitutes that are able to replace current autologous and allogeneic grafting procedures for the repair of diseased or damaged skeletal tissues. Mesenchymal stem cells (MSCs), found predominantly in the bone marrow, are able to differentiate into osteogenic, chondrogenic, adipogenic and tenogenic lineages, thus offering considerable therapeutic potential for tissue engineering applications. However, invasive extraction procedures and insufficient viable cell yields have necessitated the identification of alternative tissue sources of MSCs. Growing evidence suggests that umbilical cord blood (UCB) contains a population of rare MSCs that are able to undergo multilineage differentiation.The aim of this proposal is optimise the isolation and expansion of MSCs from human UCB (CB-MSCs). The differentiation capacity of CB-MSCs will be examined, with a specific focus on osteogenesis. The CB-MSCs will be characterised by genomic, proteomic and bioimpedance profiling and compared to MSCs isolated from human bone marrow as well as embryonic stem cells. Full bioinformatics integration of datasets will identify specific and/or novel signalling factors associated with CB-MSCs. The immunophenotype and alloreactivity of CB-MSCs will be determined. Comparative analyses of the population doubling times, telomere length and telomerase activity will identify the lifespan of CB-MSCs. Novel expansion techniques will be combined with scale-up procedures and the generation of CB-MSC lines for banking using optimised cryopreservation protocols. In vitro and in vivo biocompatibility assays using a range of biomimetic scaffolds will be exploited, complementing in vivo homing and engraftment models. Our integrated approach using complementary expertise will provide a timely and thorough evaluation of CB-MSCs and define appropriate routes for their therapeutic implementation. Dziedzina nauki medical and health sciencesclinical medicinesurgerymedical and health sciencesmedical biotechnologytissue engineeringmedical and health sciencesbasic medicineimmunologymedical and health sciencesmedical biotechnologycells technologiesstem cellsmedical and health sciencesclinical medicinetransplantation Program(-y) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Temat(-y) LSH-2004-1.2.4-6 - Exploring the potential of human umbilical cord blood stem cells for application other than allogeneic haematopoietic transplantations Zaproszenie do składania wniosków FP6-2004-LIFESCIHEALTH-5 Zobacz inne projekty w ramach tego zaproszenia System finansowania STREP - Specific Targeted Research Project Koordynator UNIVERSITY OF YORK Wkład UE Brak danych Adres Heslington YORK Zjednoczone Królestwo Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych Uczestnicy (9) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko UNIVERSITY HOSPITAL OF ODENSE Dania Wkład UE Brak danych Adres Kloevervaenget 6 ODENSE Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych UNIVERSITY OF SOUTHERN DENMARK, ODENSE Dania Wkład UE Brak danych Adres Campusvej 55 ODENSE Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V Niemcy Wkład UE Brak danych Adres Hansastraße 27C MÜNCHEN Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych UNIVERSITY OF NOTTINGHAM Zjednoczone Królestwo Wkład UE Brak danych Adres University Park NOTTINGHAM Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych RUPRECHT-KARLS-UNIVERSITÄT HEIDELBERG Niemcy Wkład UE Brak danych Adres Seminarstrasse 2 HEIDELBERG Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych ANGEL BIOTECHNOLOGY LTD Zjednoczone Królestwo Wkład UE Brak danych Adres 50 Broadway LONDON Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych ECBIO - INVESTIGAÇÃO E DESENVOLVIMENTO EM BIOTECNOLOGIA, S.A. Portugalia Wkład UE Brak danych Adres Lab 4.11, Edif IBET/ITQB, Av República OEIRAS Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych REGENTEC LTD Zjednoczone Królestwo Wkład UE Brak danych Adres BioCity Nottingham, Pennyfoot Street NOTTINGHAM Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych ANGEL BIOTECHNOLOGY HOLDINGS PLC Zjednoczone Królestwo Wkład UE Brak danych Adres BROADWAY 50 LONDON Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych
