Objetivo Despite the enormous potential of using transgenesis for gene function analysis and gene therapy, little is known about mechanisms of gene integration in eukyotic cells. Transgene integration into the chromomsomes of living cells can occur either randomly or targeted by homologous recombination. The later type of integration is the most useful, because it allows precisely deleting or modifying sequences at defined chromosomal positions. A consortium is formed to study gene integration by recombination in a number of different model organisms.As DNA repair is conserved during evolution, a comparative genomics approach is proposed to discover evolutionary conserved principles of gene integration. An explicit goal is to understand why targeted integration occurs efficiently in some eukaryotic cells, but not in others. One explanation to be investigated is that the ratios of targeted to random integration reflects to the balance between DNA double-strand break repair byhomologous recombination and end-joining. Other areas of research will relate to site-specific integration by AdenoAssociatedViruses, T-elements, serine recombinases. The insight into mechanisms of gene integration will be used to increase the efficiency of targeted gene integration in cells, where targeted integration events are currently hard to detect. This might be achieved either by modification of the gene constructs, their transfer into the cell nucleus or by regulating the expression of transacting recombination factors. As gene targeting is theonly way to precisely modify the genetic blueprint of living cells in vivo, improvements of this technique offer immense opportunities not only for basic research, but also for biotechnology and gene therapy. This ranges from fine-tuning protein production in plants and bioreactors to gene therapy for autosomal dominant defects. Two companies are included in the proposal to capture these opportunities and reenforce the com. Ámbito científico medical and health sciencesmedical biotechnologygenetic engineeringgene therapynatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticschromosomesnatural sciencesbiological sciencesgeneticsgenomes Palabras clave Targeted gene integration homologous and site specific recombination Programa(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Tema(s) LSH-2002-1.1.0-1 - Topics for Specific Targeted Research Project/CA in the area of Fundamental knowledge and basic tools for functional genomics in all organisms Convocatoria de propuestas FP6-2002-LIFESCIHEALTH Consulte otros proyectos de esta convocatoria Régimen de financiación STREP - Specific Targeted Research Project Coordinador GSF - FORSCHUNGSZENTRUM FUER UMWELT UND GESUNDHEIT GMBH Aportación de la UE Sin datos Dirección Ingolstaedter Landstrasse 1 NEUHERBERG Alemania Ver en el mapa Coste total Sin datos Participantes (7) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo THE UNIVERSITY OF NOTTINGHAM Reino Unido Aportación de la UE Sin datos Dirección University Park NOTTINGHAM Ver en el mapa Coste total Sin datos FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY VZW Bélgica Aportación de la UE Sin datos Dirección Rijvisschestraat 120 ZWIJNAARDE Ver en el mapa Coste total Sin datos COMMISSARIAT A L'ENERGIE ATOMIQUE Francia Aportación de la UE Sin datos Dirección 31-33 Rue de la Federation PARIS Ver en el mapa Coste total Sin datos CISTRONICS CELL TECHNOLOGY GMBH Suiza Aportación de la UE Sin datos Dirección Einsteinstrasse 1-5 ZURICH Ver en el mapa Coste total Sin datos INSTYTUT BIOCHEMII I BIOFIZYKI - POLSKIEJ AKADEMII NAUK Polonia Aportación de la UE Sin datos Dirección Pawinskiego 5a WARSZAWA Ver en el mapa Coste total Sin datos MAX PLANCK GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. Alemania Aportación de la UE Sin datos Dirección Hofgartenstrasse 8 MUENCHEN Ver en el mapa Coste total Sin datos UNIVERSITAET ZUERICH Suiza Aportación de la UE Sin datos Dirección Raemistrasse 71 ZURICH Ver en el mapa Coste total Sin datos
