Objetivo The nematode Caenorhabditis elegans is a widely appreciated, powerful platform in which to study important biological mechanisms related to human health. More than 65% of human disease genes have homologues in the C. elegans genome, and essential aspects of mammalian cell biology, neurobiology and development are faithfully recapitulated in this organism. We propose to develop cutting-edge tools and resources that will facilitate modeling of human pathologies in C. elegans, and advance our understanding of animal development and physiology. Specifically, we will optimize and automate existing transposon-mediated mutagenesis methodologies based on the Mos1 transposable element, in addition to developing alternatives using the Minos transposon system. We will exploit these tools to generate and evaluate a collection of transposon-tagged mutants, aiming to cover the complete genome of approximately 20,000 genes. Further, we will establish the infrastructure necessary for managing and maintaining this resource. To achieve these goals, we have assembled a group of experts with pioneering contributions to transposon and genomics technologies in C. elegans. Our partner, Union Biometrica NV, European Scientific Operations, is a company specializing on platform technology development and will provide solutions essential for the automation and streamlining of the processes involved in generating libraries of mutant strains. The final product of our focused project-a comprehensive collection of transposon-tagged alleles-together with the acquisition of efficient transposon-based tools for mutagenesis and transgenesis in C. elegans, should be of great value to the European and international scientific community. Our efforts will strengthen European research in the strategic area of functional genomics and yield a wealth of information on gene function, immediately relevant to pharmaceutical R&D. Ámbito científico natural sciencesbiological sciencesneurobiologysocial sciencessociologyindustrial relationsautomationnatural sciencesbiological sciencescell biologymedical and health sciencesbasic medicinepathologynatural sciencesbiological sciencesgeneticsgenomes Palabras clave Cacnorhabditis elegans Functional Genomics Gene Knock Gene Knock-Out Heterologous Transposition Programa(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Tema(s) LSH-2002-1.1.0-1 - Topics for Specific Targeted Research Project/CA in the area of Fundamental knowledge and basic tools for functional genomics in all organisms Convocatoria de propuestas FP6-2002-LIFESCIHEALTH Consulte otros proyectos de esta convocatoria Régimen de financiación STREP - Specific Targeted Research Project Coordinador FOUNDATION FOR RESEARCH AND TECHNOLOGY HELLAS Aportación de la UE Sin datos Dirección Vassilika Vouton 1527 HERAKLION Grecia Ver en el mapa Coste total Sin datos Participantes (5) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE Francia Aportación de la UE Sin datos Dirección 101 Rue de Tolbiac PARIS Ver en el mapa Coste total Sin datos CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE Francia Aportación de la UE Sin datos Dirección Rue Michel Ange 3 287 PARIS Ver en el mapa Coste total Sin datos MAIA SCIENTIFIC Bélgica Aportación de la UE Sin datos Dirección Cipalstraat, 3 GEEL Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos UNIVERSITY OF BRISTOL Reino Unido Aportación de la UE Sin datos Dirección Senate House, Tyndall Avenue BRISTOL Ver en el mapa Coste total Sin datos UNIVERSITE CLAUDE BERNARD LYON 1 Francia Aportación de la UE Sin datos Dirección Bld du 11 novembre 1918, 43 VILLEURBANNE Ver en el mapa Coste total Sin datos
