Objetivo Results will give new and important insight into how the eukaryotic genome in general, and the human genome in particular, operate inside the living cell. This program will lay the groundwork for understanding how, beyond the regulation at the individual gene level, large-scale chromatin structure affects the complex gene regulation networks in normal and deceased cells. Understanding molecular mechanisms that underlie regulation of this large set of genes is a key target in modern medical sciences and biotechnology. Our knowledge about gene regulation at the single gene and the epigenetic level is rapidly expanding.However, our understanding of the role in gene control of the three-dimensional folding of the genome in the cell nucleus, is still poorly understood; this despite the fact that considerable evidence is available showing that large-scale chromatin organization plays an important role in gene control in higher eukaryotes. 3DGENOME has the ambition to force a breakthrough in our understanding of the relationship between the function of the genome and its dynamic 3D structure in the cell. To this end we will analyse the 3D structure of the human genome in the cell nucleus and relate this to its transcriptional activity. Structural analysis is carried out by fluorescent in situ hybridization (FISH) in combination with advanced 3D light microscopy. Large-scale chromatin structure of four selected chromosomes (11, 17, 18 and X) will be determined in three different cell types, differing in gene expression. Gene activity along the chromosomal fibre will be read from the human transcriptome map and correlated with structure. Work on human cells will be expanded to mouse and Drosophila,aiming at establishing causal and evolutionary conserved relationships between 3D genome structure and genome activity. Ámbito científico natural sciencesbiological sciencesgeneticsDNAnatural sciencescomputer and information sciencescomputational sciencenatural sciencesphysical sciencesopticsmicroscopyconfocal microscopynatural sciencesbiological sciencesgeneticschromosomesnatural sciencesbiological sciencesgeneticsgenomeseukaryotic genomes Palabras clave 3D structure Fluorescent in situ hybridization gene expression high throughput high throughput imaging analysis imaging analysis Programa(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Tema(s) LIFESCIHEALTH-1.1 - Fundamental knowledge and basic tools for functional genomics in all organisms Convocatoria de propuestas FP6-2002-LIFESCIHEALTH Consulte otros proyectos de esta convocatoria Régimen de financiación STREP - Specific Targeted Research Project Coordinador UNIVERSITEIT VAN AMSTERDAM Aportación de la UE Sin datos Dirección Spui 21 AMSTERDAM Países Bajos Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos Participantes (7) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo DEUTSCHES KREBSFORSCHUNGSZENTRUM Alemania Aportación de la UE Sin datos Dirección Im Neuenheimer Feld 280 10 19 49 HEIDELBERG Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE Francia Aportación de la UE Sin datos Dirección Rue Michel Ange n°3 PARIS Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos ACADEMISCH ZIEKENHUIS BIJ DE UNIVERSITEIT VAN AMSTERDAM Países Bajos Aportación de la UE Sin datos Dirección Meibergdreef 9, 1105 AZ AMSTERDAM Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos SCIENTIFIC VOLUME IMAGING BV Países Bajos Aportación de la UE Sin datos Dirección Alexanderlaan 14 HILVERSUM Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos BIOFYZIKALNI USTAV AKADEMIE VED CESKÉ REPUBLIKY Chequia Aportación de la UE Sin datos Dirección Kralovopolska 135 BRNO Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos LUDWIG-MAXIMILIANS UNIVERSITÄT MUENCHEN Alemania Aportación de la UE Sin datos Dirección Geschwister Schollplatz 1 MUNICH Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos RUPRECHT-KARLS-UNIVERSITÄT HEIDELBERG Alemania Aportación de la UE Sin datos Dirección Seminarstrasse 2 HEIDELBERG Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos