Objetivo We will use systems biology approaches to model RNA metabolism in yeast to aid understanding of these complex cellular pathways. In order to develop kinetic models, we propose to quantify RNA precursors and directly determine their rates of production as w ell as their processing and degradation through the various post-transcriptional pathways. Starting with ab-initio models describing the processing and degradation of yeast pre-messenger RNAs and pre-ribosomal RNAs, we will produce two comparable mathemati cal representations and populate the parameters using quantitative experimental data. Manipulation of the parameters will permit predictions to be made about the behaviour of the systems. These will be tested experimentally, using yeast mutants that block specific steps, or chemical inhibitors identified in high-throughput screens. Imaging techniques will be refined to visualise individual transcripts to determine whether the population data reflect the situation in individual cells. Comparison of the perfo rmance of the two models should provide further insights and enrich our understanding of both pathways. In addition, modelling precursor RNA processing in yeast will be of great benefit for understanding of these pathways in human cells, which are less ame nable to direct experimentation, and their significance for human genetic disorders. Ámbito científico natural sciencesbiological sciencesgeneticsRNA Programa(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Tema(s) LSH-2002-1.1.0-1 - Topics for Specific Targeted Research Project/CA in the area of Fundamental knowledge and basic tools for functional genomics in all organisms Convocatoria de propuestas FP6-2004-LIFESCIHEALTH-5 Consulte otros proyectos de esta convocatoria Régimen de financiación STIP - Specific Targeted Innovation Project Coordinador THE UNIVERSITY OF EDINBURGH Aportación de la UE Sin datos Dirección Old College EDINBURGH Reino Unido Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos Participantes (5) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE DELEGATION LANGUEDOC-ROUSSILLON Francia Aportación de la UE Sin datos Dirección Route de Mende N°1919 MONTPELLIER Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos COMPUGEN LTD. Israel Aportación de la UE Sin datos Dirección 72 Pinchas Rosen st. TEL AVIV Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos UNIVERSITAT ZURICH Suiza Aportación de la UE Sin datos Dirección Raemistrasse 71 ZURICH Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos WARSAW UNIVERSITY Polonia Aportación de la UE Sin datos Dirección Krakowskie Przedmiescie 26/28 WARSAW Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos INSTITUTE FOR SYSTEMS BIOLOGY SPB COMPANY LIMITED Rusia Aportación de la UE Sin datos Dirección 80-48 Toreza Avenue SAINT-PETERSBURG Ver en el mapa Coste total Sin datos
