Cel We will use systems biology approaches to model RNA metabolism in yeast to aid understanding of these complex cellular pathways. In order to develop kinetic models, we propose to quantify RNA precursors and directly determine their rates of production as w ell as their processing and degradation through the various post-transcriptional pathways. Starting with ab-initio models describing the processing and degradation of yeast pre-messenger RNAs and pre-ribosomal RNAs, we will produce two comparable mathemati cal representations and populate the parameters using quantitative experimental data. Manipulation of the parameters will permit predictions to be made about the behaviour of the systems. These will be tested experimentally, using yeast mutants that block specific steps, or chemical inhibitors identified in high-throughput screens. Imaging techniques will be refined to visualise individual transcripts to determine whether the population data reflect the situation in individual cells. Comparison of the perfo rmance of the two models should provide further insights and enrich our understanding of both pathways. In addition, modelling precursor RNA processing in yeast will be of great benefit for understanding of these pathways in human cells, which are less ame nable to direct experimentation, and their significance for human genetic disorders. Dziedzina nauki natural sciencesbiological sciencesgeneticsRNA Program(-y) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Temat(-y) LSH-2002-1.1.0-1 - Topics for Specific Targeted Research Project/CA in the area of Fundamental knowledge and basic tools for functional genomics in all organisms Zaproszenie do składania wniosków FP6-2004-LIFESCIHEALTH-5 Zobacz inne projekty w ramach tego zaproszenia System finansowania STIP - Specific Targeted Innovation Project Koordynator THE UNIVERSITY OF EDINBURGH Wkład UE Brak danych Adres Old College EDINBURGH Zjednoczone Królestwo Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych Uczestnicy (5) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE DELEGATION LANGUEDOC-ROUSSILLON Francja Wkład UE Brak danych Adres Route de Mende N°1919 MONTPELLIER Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych COMPUGEN LTD. Izrael Wkład UE Brak danych Adres 72 Pinchas Rosen st. TEL AVIV Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych UNIVERSITAT ZURICH Szwajcaria Wkład UE Brak danych Adres Raemistrasse 71 ZURICH Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych WARSAW UNIVERSITY Polska Wkład UE Brak danych Adres Krakowskie Przedmiescie 26/28 WARSAW Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych INSTITUTE FOR SYSTEMS BIOLOGY SPB COMPANY LIMITED Rosja Wkład UE Brak danych Adres 80-48 Toreza Avenue SAINT-PETERSBURG Zobacz na mapie Koszt całkowity Brak danych
