Obiettivo The use of formalin-fixed specimens in genetic studies has been limited due to damage of the nucleic acids through contact with the formalin. However, recent developments in extraction and repair techniques have increased both the yields and quality of the DNA and RNA sequences that can be retrieved from such specimens. Additionally, the invention of advanced Multi-Plex PCR techniques has made it possible to quickly screen a large number of genomic markers. Thus, for the first time, formalin-fixed specimens have become available to large-scale genetic analyses.This proposal will enable the principal applicant to become an expert on the retrieval and analyses of nucleic acid from formalin fixed specimens. Based at the University of Copenhagens Centre for Anci ent DNA and Evolutionary Biology, the applicant will be trained by a team of expert mentors in the use, design and theory behind modern high-throughput genetic screening assays for ancient and contemporary DNA. He will also be trained in advanced statistic al and analytical techniques required to develop and analyze the data from genetic association studies. The applicant will apply these techniques to anonymous, HIV-1 infected, fixed biopsy and autopsy specimens that date to the early period of the epidemic in Northern Europe (and lt 1990). Thereby, he will investigate the dynamics of the evolutionary interaction between HIV-1 and the human genome, principally focusing on association studies between the virus and the Human Leukocyte Antigen (HLA) system. The outcomes of this project will not only confirm current hypotheses, and shed new insights, into the model HIV-1-human system, but will develop the tools through which future investigations that require high-throughput screening techniques of fixed specimen s can be achieved. Campo scientifico natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesevolutionary biologynatural sciencesbiological sciencesgeneticsRNAnatural sciencesbiological sciencesgeneticsgenomes Parole chiave 1 Association Autopsy Biopsy DNA Formalin Fixed HIV HLA High Minisequencing Multiplex PCR Nucleic Acids Paraffin Embedded RNA Throughput Genetic Analyses Programma(i) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Argomento(i) MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF) Invito a presentare proposte FP6-2004-MOBILITY-5 Vedi altri progetti per questo bando Meccanismo di finanziamento EIF - Marie Curie actions-Intra-European Fellowships Coordinatore UNIVERSITY OF COPENHAGEN Contributo UE Nessun dato Indirizzo Norregade 10 COPENHAGEN Danimarca Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato
