Objectif Bacterial pathogens encountering host cells employ several attachment factors, which promote direct contact and trigger distinct signalling pathways in the host. The stimulation of particular signalling pathways subverts the eucaryotic cell and enables sur vival and persistence of bacteria within the host. Identification and characterisation of communicative tools between the bacteria and the host cells should lead to a better understanding of the infectious process and the development of new anti-infectious treatment. Pseudomonas aeruginosa is a Gram-negative bacterium causing nosocomial infections, wound infections, and lethal infections in Cystic Fibrosis patients. In the context of this proposal we aim at characterising the function of proteins encoded wi thin the P. aeruginosa cupB cluster, particularly that of the putative adhesins cupB6 and cupB5. The approach will employ cupB promoter studies to identify signals stimulating adhesion to eucaryotic cells. Expression levels of host cellular components upon infection with the wild type or cupB mutant strains will be analysed by high density DNA arrays. Cell lines with the Cystic fibrosis (CF) phenotype and Non CF cell lines will be used. Such an approach could be extended to other adhesins specific for epith elial cell - binding. The results will provide a specific profiling of gene expression and signalling pathways in response to the presence of particular adhesion factors in different P. aeruginosa strains. Champ scientifique natural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteins Mots‑clés Pseudomonas adherence bacteria bacteria-host-interaction host interaction microarray Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Thème(s) MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF) Appel à propositions FP6-2002-MOBILITY-5 Voir d’autres projets de cet appel Régime de financement EIF - Marie Curie actions-Intra-European Fellowships Coordinateur CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE Contribution de l’UE Aucune donnée Adresse Rue Michel Ange 3 PARIS France Voir sur la carte Coût total Aucune donnée
