Cel The prominent role of water in the plant life provides the motivation for a number of scientific groups to investigate the role of water channels, aquaporins (AQPs), in the plant water relations. AQPs are present in all the living organisms that have been researched to date. They transport water as well as small neutral solutes across the cell membranes.The understanding of the AQP molecular functionality and structure progressed considerably during the last decade. In spite of that, the mechanism by which the AQP activity is being regulated at the plant cell level is still poorly understood. Our main objectives are to achieve a better understanding of the mechanism controlling the water permeability at the level of a single cell via the regulation of its plasma membrane AQPs, and to correlate this mechanism with the whole plant-water relationship.Two major mechanisms are likely to be involved: gating (conformational changes of the AQPs) and trafficking (shuttling of small vesicles rich in AQPs). Specific AQ P gating models in plants are based mainly on data obtained after plant AQP expression in Xenopus oocytes (e.g. gating behaviour of AtPIP2;2. controlled by the cytosolic pH, of Soybean nodulin 26 controlled by phosphorylation). Trafficking models in plants are based on proteins other than AQPs (e.g. Auxin transporter PINI) or on mammalian AQP2 and AQP4 trafficking models. Identifying similar mechanisms directly involved in the regulation of plant cell water permeability should enable us to achieve these objectives.To this end, we plan to investigate the plant AQP activity using specific chemicals known to affect the cellular concentrations of protons or Ca2+, second messenger ions involved in many regulations processes. Dziedzina nauki natural sciencesbiological sciencesgeneticsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsagricultural sciencesagriculture, forestry, and fisheriesagriculturenatural sciencesbiological sciencesbotany Słowa kluczowe BMS cells MIP Pf gating green fluorescent protein membrane osmotic permeability plant tobacco trafficking transgene expression transport models water channels Program(-y) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Temat(-y) MOBILITY-4.1 - Marie Curie European Reintegration Grants (ERG) Zaproszenie do składania wniosków FP6-2002-MOBILITY-11 Zobacz inne projekty w ramach tego zaproszenia System finansowania ERG - Marie Curie actions-European Re-integration Grants Koordynator THE HEBREW UNIVERSITY OF JERUSALEM. Wkład UE Brak danych Adres The Authority for Research and Development, Edmond J. Safra. Campus, Givat Ram JERUSALEM Izrael Zobacz na mapie Koszt całkowity Brak danych
