Objectif Novel methodology involving the specific in vivo biotinylation of tagged proteins in mammalian cells has been developed. The proposed project entails the application of this methodology as a one step high affinity approach in purifying novel protein complexes of the hybrid proteins formed from translocations in Chronic MyeloProliferative Disorders (CMPDs).The CMPDs, a category of leukaemias, are characterized by uncontrolled proliferation of one or several cell types of the myeloid or haematopoietic lineages. Translocations found in CMPDs create hybrid genes, producing hybrid proteins functioning as activated tyrosine kinases that trigger the uncontrolled cell proliferation. The proposed project will focus on the purification of complexes of two such hybrid proteins: BCR-FGFR1 and BCR-PDGFRA.A bio-tag will be fused to the specific proteins and will be co-expressed in mammalian cells with a bacterial biotin transferase, which recognises and specifically biotinylates the tagged protein. Complexes of biotinylated proteins will be purified by binding to streptavidin and bound proteins will be eluted and identified by mass spectrometry. This project will elucidate the molecular mechanisms causing the CMPDs and will provide insights in the field of proteomics for these disorders.The future goal will be to translate this knowledge into new strategies for clinical management, at both the diagnostic and therapeutic level (i.e. identification of novel inhibitors of the proteins identified for treatment of CMPDs and potential siRNAs assays to inhibit the hybrid proteins or their partners). Champ scientifique natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesbiological sciencesgeneticsDNAmedical and health sciencesclinical medicineoncologyleukemianatural scienceschemical sciencesanalytical chemistrymass spectrometry Mots‑clés Chronic MyeloProliferative Disorders biotinylation complex purification hybrid proteins tyrosine kinases Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Thème(s) MOBILITY-4.1 - Marie Curie European Reintegration Grants (ERG) Appel à propositions FP6-2002-MOBILITY-11 Voir d’autres projets de cet appel Régime de financement ERG - Marie Curie actions-European Re-integration Grants Coordinateur FOUNDATION FOR BIOMEDICAL RESEARCH OF THE ACADEMY OF ATHENS Contribution de l’UE Aucune donnée Adresse Soranou tou Efesiou 4 ATHENS Grèce Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée