Objectif The DNA of the eukaryotic cell is accommodated by the structure of chromatin. To administrate the genetic information the cell employs multiple mechanisms regulating protein-DNA transactions in the context of chromatin. Research of the past two decades has demonstrated that chromatin undergoes dynamic changes depending on its functional state: The major chromatin components, the histones, are subject to covalent modifications by specific enzymatic activities, furthermore multi-protein complexes have been de scribed that structurally alter chromatin in ATP-dependent processes. A detailed molecular analysis eventually requires isolation of pure, natural chromatin closely reflecting the in vivo situation. We have established a novel technique to purify single-copy genes from chromosomal loci in the yeast Saccharomyces cerevisiae to near homogeneity, conserving the chromatin structure of distinct transcriptional states.Future research pursues two lines of investigations:(i) Determination of structure and composition of natural chromatin in different functional states by electron microscopy and mass spectrometry(ii) Description of the functional interplay between chromatin remodelling and transcription using isolated natural chromosomal domains and purified protein factor in vitro Champ scientifique natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesphysical sciencesopticsmicroscopyelectron microscopynatural sciencesbiological sciencesgeneticsRNAnatural scienceschemical sciencesanalytical chemistrymass spectrometry Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Thème(s) MOBILITY-4.2 - Marie Curie International Reintegration Grants (IRG) Appel à propositions FP6-2002-MOBILITY-12 Voir d’autres projets de cet appel Régime de financement IRG - Marie Curie actions-International re-integration grants Coordinateur UNIVERSITY OF REGENSBURG Contribution de l’UE Aucune donnée Adresse Universitaetsstrasse 31 Regensburg Allemagne Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée