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European Network on selection and analysis of protein-protein Interactions

Objective

Networks of protein-protein interactions are central to cell and developmental biology and of great importance in molecular medicine. Given the potential importance of protein-protein interactions to healthcare, wealth creation and the biotech industry the re is an urgent need to train young researchers in the latest technologies aimed at identifying and dissecting such interactions by the very latest in high-throughput approaches, bio-informatics and rigorous biophysical analysis. We will train early-stage and experienced researchers to use new bio-informatics tools to direct high-throughput screening (HTS) for new protein-protein interactions. Network "hubs" or "nodes" (i.e. proteins/protein families that interact crucially with one or many other partners in the network) will be identified and used as baits in HTS.

Based on previous collaborative, published work, we will develop an in vitro selection system for protein-protein interactions by in vitro compartmentalisation (IVC). IVC relies on dispersing aqueous reactions into microscopic water-in-oil emulsion droplets ( ~5 fl) enabling selection of 10e10 pairs of interacting proteins. Hence, all ~10e9 possible pair wise binding interaction of proteins encoded by the human genome are possible in a more economical format than existing HTS techniques.

A novel system for covalently labelling proteins will be used to label and cross-link proteins in vivo and to create protein micro-arrays. Kinetic and thermodynamic characterisation of identified protein-protein interact ions will be performed using a variety of techniques including a novel acoustic biosensor. Most importantly, we aim at an integrated approach where the bio-informatics tools we develop will be used to direct the HTS efforts. In turn, results obtained from t he screening will help validate and tune our newly-developed computational tools, while detailed mechanistic studies will provide insight into the physiological role of newly-identified interacting pairs.

Call for proposal

FP6-2004-MOBILITY-1
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Funding Scheme

RTN - Marie Curie actions-Research Training Networks
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Coordinator

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIGE
Address
The Old Schools, Trinity Lane
Cambridge
United Kingdom

Participants (10)

COVALYS BIOSCIENCES AG
Switzerland
Address
Benkenstrasse 254
Witterswil
ECOLE POLYTECHNIQUE FÃ‘Æ Ã Â©DÃ‘Æ Ã Â©RALE DE LAUSANNE
Switzerland
Address
Ecublens
Lausanne
FOUNDATION FOR RESEARCH AND TECHNOLOGY-HELLAS - FORTH
Greece
Address
Vassilika Vouton
Heraklion, Crete
HELSINGIN YLIOPISTO
Finland
Address
Yliopistonkatu 4
Helsingin Yliopisto, Helsinki
MAX-PLANCK-GESELLSCHAFT ZUR FÃ‘Æ Ã Â¶RDERUNG DER WISSENSCHAFTEN E.V.
Germany
Address
Hofgartenstrasse 8
Mã‘æ à ¼nchen
UNIVERSITAT ULM
Germany
Address
Helmholtzstrasse 16
Ulm
UNIVERSITE LOUIS PASTEUR
France
Address
4, Rue Blaise Pascal
Strasbourg
UNIVERSITY OF YORK
United Kingdom
Address
Heslington
York
WEIZMANN INSTITUTE OF SCIENCE
Israel
Address
Herzelstreet 2
Rehovot
WESTFÄLISCHE WILHELMS-UNIVERSITÄT MÜNSTER
Germany
Address
Schlossplatz 2
Münster