Skip to main content

Flow cytometry for fast and sensitive diagnosis and follow-up of haematological malignancies

Objective

Laboratory diagnostics of haematological malignancies has three major applications: establishing the diagnosis, prognostic classification, and evaluation of treatment effectiveness. Over the past decade, several molecular techniques have brought new insights into classification and monitoring of treatment effectiveness. However, they have several major disadvantages: they are time consuming, not applicable in all categories of patients, and cannot focus on cellular subpopulations without purification steps. These limitations can be overcome by innovations in flow cytometry. Flow cytometric immunophenotyping is the sole technique that fulfils the requirements of high speed, broad applicability at diagnosis and follow-up, and accurate focusing on the malignant cell population using membrane-bound and intracellular proteins as targets. The required innovative steps concern development of novel antibodies, novel immunobead technology, novel flow cytometry software, and novel ³ 6-colour immunostaining protocols, which are all key objectives of the EuroFlow consortium. This needs a multidisciplinary translational research approach using cutting edge technologies and biological data arising from genomic research, which can be addressed most successfully via close collaboration between industry and academia. Consequently, the EuroFlow consortium will consist of two SME's and 8 diagnostic research groups, which are regarded as experts in the fields of flow cytometric and molecular diagnostics. Together the 10 participants have sufficient complementarity and congruence to cover all aspects of development, standardisation, and validation of highly sensitive tests for diagnosis and follow-up. The successful implementation of the proposal and the dissemination of the results to European haematological laboratories should result in health care progress through more precise diagnosis and individualised treatment as well as in strengthening o

Call for proposal

FP6-2004-LIFESCIHEALTH-5
See other projects for this call

Funding Scheme

STREP - Specific Targeted Research Project

Coordinator

ERASMUS UNIVERSITY MEDICAL CENTER ROTTERDAM
Address
Dr. Molenwaterplein 50
PO BOX 1738 Rotterdam
Netherlands

Participants (9)

UNIVERSITY OF SALAMANCA
Spain
Address
Edificio De Rectorado, Patio De Escuelas, 1
Salamanca
DYNOMICS B.V.
Netherlands
Address
Borneokade 279
Amsterdam
CYTOGNOS S.L.
Spain
Address
Polígono De La Serna Nave 9
Santa Marta De Tormes - Salamanca
INSTITUTO DE MEDICINA MOLECULAR
Portugal
Address
Av. Prof. Egas Moniz
Lisboa
UNIVERSITY HOSPITAL SCHLESWIG-HOLSTEIN
Germany
Address
Brunswiker Str. 10
Kiel
ASSISTANCE PUBLIQUE-HOPITAUX DE PARIS
France
Address
3 Avenue Victoria
Paris
UNIVERSITY OF LEEDS
United Kingdom
Address
Woodhouse Lane
Leeds
CHARLES UNIVERSITY
Czechia
Address
Ovocny Trh 5
Prague
MEDICAL UNIVERSITY OF SILESIA
Poland
Address
14 Warszawska
Katowice