Objectif In this project we propose to study the relation between the cell cycle and dormancy development in Mycobacterium tuberculosis (Mtb). The final aim is to increase the knowledge on dormancy development and to identify potential candidates for the development of antitubercular drugs specifically active in dormant bacilli. Mtb is a facultative intracellular pathogen able to infect the human lung for decades without showing any symptom. Following immunity suppression caused by age, diseases, or malnutrition, tubercle bacilli can reactivate to give overt disease. About one third of the human population is estimated to be affected by latent tuberculosis representing a huge reservoire for the infection. The lack of drugs efficiently active on dormant bacteria is a major problem in fighting this important disease. Conditional mutants unable to express important cell cycle regulators will be constructed and used to study their role in i) global regulation of gene expression; ii) cell cycle proteins cytolocalization; iii) latency development in vitro and in vivo. Additionally, a transposon insertion library will be used to identify new mycobacterial genes essential for survival to prolonged stationary phase, and their expression will be measured during progressive and latent infection. Champ scientifique medical and health sciencesbasic medicinepharmacology and pharmacydrug discoverynatural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesclinical medicinepneumologytuberculosismedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsvaccines Programme(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Thème(s) LSH-2005-2.3.0-4 - New approaches for research into host/vector-pathogen interaction for HIV/AIDS, malaria and tuberculosis LIFESCIHEALTH - Life sciences, genomics and biotechnology for health Appel à propositions FP6-2005-LIFESCIHEALTH-6 Voir d’autres projets de cet appel Régime de financement STREP - Specific Targeted Research Project Coordinateur UNIVERSITÀ DEGLI STUDI PADOVA Contribution de l’UE Aucune donnée Adresse Via 8 Febbraio, 2 PADOVA Italie Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée Participants (4) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS Espagne Contribution de l’UE Aucune donnée Adresse SERRANO 117 MADRID Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée INSERM France Contribution de l’UE Aucune donnée Adresse 156 rue de Vaugirard PARIS Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée INSTITUTO VENEZOLANO DE INVESTIGACIONES CIENTÍFICAS Venezuela Contribution de l’UE Aucune donnée Adresse Km 11 Carretera Panamericana ESTADO MIRANDA Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée NATIONAL INSTITUTE OF MEDICAL SCIENCES AND NUTRITION "SALVADOR ZUBIRAN" Mexique Contribution de l’UE Aucune donnée Adresse Vasco de Quiroga NO. 15, Col. Seccion XVI MEXICO CITY Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée