Obiettivo Therapeutic vaccines targeting B cell non-Hodgkin lymphoma (NHL) idiotype (Id) represent a promising immunotherapeutic approach for a more effective control of these malignancies. A broad use of Id-based vaccination, however, is hampered by the complexity and costs due to the individualized production of these vaccines. Recent evidence suggests that these limitations may be overcome. In fact, distinct sets of stereotyped immunoglobulins have been identified in various B-NHL, suggesting that a much higher frequency of shared Id exists among patients than appreciated previously. We found that the VK3-20 protein, frequently expressed by HCV-related B-NHL, induces specific T cell responses that cross-react against related VK proteins commonly used by different B cell lymphoproliferations. These findings provide the rationale to develop recombinant vaccines using Id shared by different B cell malignancies. Through the complementary and synergistic work of academic partners and three SMEs, we plan to develop and produce optimized recombinant Id vaccines for subset-specific immunotherapy. A database of VH and VL sequences expressed by low-grade B-NHL and chronic lymphocytic leukemias will be established and analyzed to identify subgroups of tumours expressing molecularly correlated Id proteins. Selected Id proteins will be characterized for their immunogenicity and, particularly, for the ability to induce 'cross-reactive¿ immune responses against related Id proteins. B and T cell epitopes will be identified using innovative approaches (pentamer libraries, MATRIX-SCAN) and dedicated assays for immunomonitoring will be developed. Moreover, optimized versions of selected Id vaccines will be produced using new strategies (Gly-Ala repeat insertion, peptide sequence optimization) and validated in animal models. New adjuvants and delivery systems for improved Id vaccine formulations and administration will be also evaluated and validated. The most promising Id proteins will be... Campo scientifico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health scienceshealth sciencesinfectious diseasesRNA virusesHIVmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsvaccinesmedical and health sciencesbasic medicineimmunologyimmunotherapymedical and health sciencesclinical medicineoncologyleukemia Programma(i) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Argomento(i) LSH-2005-2.2.0-7 - Innovative technological approaches for cancer therapy Invito a presentare proposte FP6-2005-LIFESCIHEALTH-7 Vedi altri progetti per questo bando Meccanismo di finanziamento STREP - Specific Targeted Research Project Coordinatore CENTRO DI RIFERIMENTO ONCOLOGICO Contributo UE Nessun dato Indirizzo Via Franco Gallini 2 AVIANO PN Italia Mostra sulla mappa Costo totale Nessun dato Partecipanti (7) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto UNIVERSITETET I OSLO Norvegia Contributo UE Nessun dato Indirizzo Problemveien 5-7 OSLO Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato KAROLINSKA INSTITUTE Svezia Contributo UE Nessun dato Indirizzo Nobels Vagen STOCKHOLM Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato UNIVERSITY OF PADOVA Italia Contributo UE Nessun dato Indirizzo Via 8 febbraio 2 PADOVA Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato PEPSCANSYSTEM Paesi Bassi Contributo UE Nessun dato Indirizzo Edehertweg 15 2098 LELYSTAD Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato PROIMMUNE Regno Unito Contributo UE Nessun dato Indirizzo Magdalen Centre, Oxford Science Park OXFORD Mostra sulla mappa Costo totale Nessun dato ARETA INTERNATIONAL Italia Contributo UE Nessun dato Indirizzo Via Regina Giovanna 17 MILAN Mostra sulla mappa Costo totale Nessun dato PEPSCAN THERAPEUTICS BV Paesi Bassi Contributo UE Nessun dato Indirizzo Zuidersluisweg 2 2098 LELYSTAD Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato