CORDIS
EU research results

CORDIS

English EN

Role of Legionella pneumophila glucosyltransferases in host-pathogen interactions

Objective

Legionella pneumophila is the infectious agent of Legionnaires' disease, a serious pneumonic illness of humans. This bacterium represents a facultative intracellular parasite that invades and proliferates in many types of eukaryotic cells, including fresh water protozoans, human alveolar macrophages and epithelia. Numerous bacterial factors have been identified as crucial for intracellular infection, however for majority of these activities, their role in virulence is unclear and/or their eukaryotic substrate(s) are unknown. Recently one of the Russian applicants (Y.B.) of the collaborative project discovered for the first time and isolated from L. pneumophila a protein (Lpg1368) possessing glucosyltransferase activity, which modifies an ~50kDa eukaryotic protein substrate. Moreover, using translated amino acid sequence of lpg1368 gene as a template, other products with ~20% identity have been identified on the Legionella chromosome (Lpg2862 and Lpg1488). Importantly, all three proteins exhibited sequence similarity with the catalytic domains of clostridial glucosylating toxins.

Thus, the main objective of the current proposal includes elucidation of the role of these glucosylating enzymes in the host-pathogen interaction of L. pneumophila. For this purpose a panel of multidisciplinary approaches will be used, including those from microbiology, molecular biology, eukaryotic cell biology and pharmacology, to elucidate the properties and functions of the L. pneumophila proteins and their target(s).

Moreover, it is proposed to study the transport mechanism involved in the introduction of the bacterial enzyme into the target cells. The results obtained during the project will be of major importance both for basic biomedical science to disclose molecular mechanisms of pathogen-host interactions, as well as for applied science to generate a background for development of new methods of diagnosis, therapy and prophylaxis of legionellosis and possibly, by analogy, of other infectious diseases.

Leaflet | Map data © OpenStreetMap contributors, Credit: EC-GISCO, © EuroGeographics for the administrative boundaries

Coordinator

ALBERT-LUDWIGS UNIVERSITÄT FREIBURG

Address

AlbertstraSSE, 45
Freiburg

Germany

Administrative Contact

Klaus AKTORIES

Participants (3)

Sort alphabetically

Expand all

GAMALEYA RESEARCH INSTITUTE OF EPIDEMIOLOGY AND MICROBIOLOGY

Russia

GAMALEYA RESEARCH INSTITUTE OF EPIDEMIOLOGY AND MICROBIOLOGY

Russia

KATHOLIEKE UNIVERSITEIT LEUVEN

Belgium

Project information

Grant agreement ID: INTAS 2005-1000004-7756

  • Start date

    1 April 2006

  • End date

    30 September 2008

Funded under:

IC-INTAS

  • Overall budget:

    € 110 040

  • EU contribution

    € 150 000

Coordinated by:

ALBERT-LUDWIGS UNIVERSITÄT FREIBURG

Germany