Objectif The efficiency of DNA damage response is a subject of normal populational variations. The reduced ability to combat DNA alterations is associated with cancer susceptibility and can be attributed either to deficient DNA repair or, alternatively, to suboptimal cell death capacity. While the relationships between cancer risk and polymorphism of DNA repair genes are being intensively studied, the alternative aspect of the DNA damage response, i.e. apoptosis, has been unjustly overlooked in the research of tumour-associated gene polymorphisms.This project is aimed at testing the hypothesis whether single nucleotide polymorphisms (SNPs) associated with the reduced apoptotic capacity contribute to the increased cancer risk. The following activities will be carried out:1) Identification and validation of functional and tagging SNPs in apoptosis genes:a) Identification of already known SNPs in research publications and Internet databases;b) Validation of the occurrence of 'dubious' SNP by real-time AS-PCR genotyping;c) Search for the new SNPs in previously non-tested genes by direct DNA sequencing.2) Study on the role of apoptosis-related SNPs in cancer predisposition:a) Preliminary study of the involvement of SNP candidates in breast cancer (BC) and lung cancer (LC) susceptibility: 'comparison of extremes' approach;b) Traditionally designed, large case-control study for the promising SNP candidates. 3) Study on the functional significance of SNPs in apoptotic genes: analysis of correlation between individual genotype and the extent of apoptosis in lymphocytes exposed to DNA damaging dose of ionizing radiation.4) Comparison of the rate of DNA damage induced apoptosis in lymphocytes isolated from cancer patients vs. healthy controls.5) Comparison of radiation-induced transcriptional activation of apoptotic genes in 'high' vs. 'poor' apoptotic responders using cDNA array profiling and RT-PCR analysis.If successful, the project will significantly improve the fundamental understanding of mechanisms of cancer susceptibility as well as facilitate the development of practical approaches for the detection of at-risk individuals. Mots‑clés Epidemiology Experimental & Clinical Oncology Human Genetics Programme(s) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Thème(s) Data not available Appel à propositions Data not available Régime de financement Data not available Coordinateur LEIDEN UNIVERSITY MEDICAL CENTER Contribution de l’UE Aucune donnée Adresse ALBINUSDREEF, 2 LEIDEN Pays-Bas Voir sur la carte Coût total Aucune donnée Participants (4) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire FINNISH INSTITUTE OF OCCUPATIONAL HEALTH (FIOH) Finlande Contribution de l’UE Aucune donnée Adresse TOPELIUKSENKATU, 41 A HELSINKI Voir sur la carte Coût total Aucune donnée KAROLINSKA INSTITUTE Suède Contribution de l’UE Aucune donnée Adresse NOBELS VÄGEN, 16 STOCKHOLM Voir sur la carte Coût total Aucune donnée PETROV INSTITUTE OF ONCOLOGY Russie Contribution de l’UE Aucune donnée Adresse LENINGRADSKAYA STR., 68 ST.PETERSBURG Voir sur la carte Coût total Aucune donnée THE NORWEGIAN RADIUM HOSPITAL, INSTITUTE FOR CANCER RESEARCH Norvège Contribution de l’UE Aucune donnée Adresse ULLERNCHAUSSEEN, 70 OSLO Voir sur la carte Coût total Aucune donnée