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Cis-regulatory logic of the transcriptional control in neural stem cells

Objective

Neural stem cells (NSCs) have emerged as a major topic in neurobiology. The persistence of multipotent cells in the adult mammalian brain offers a realistic chance for the treatment of neurodegenerative diseases. It is thus crucial to understand NSCs from as many as possible angles (e. g. cellular and molecular biology), in order to better isolate and successfully manipulate them. CISSTEM presents a post-genomic systems biology approach, taking advantage of new computational and experimental tools to address the specification and maintenance of NSCs at the transcriptional/epigenetic level. CISSTEM is designed to unravel the basic principles of gene regulation in NSC, with a focus on cis-regulatory modules (CRMs). To do so we follow a multidisciplinary approach tightly interconnecting computational prediction and experimental validation in vitro and in vivo using different vertebrate models systems. Major intermediate objectives of this project are the prediction of relevant elements and the identification of the temporal, spatial and quantitative activities of predicted conserved regulatory motifs associated with NSC expressed genes. To achieve this goal, CISSTEM will i) Develop computational tools and resources for the in silico identification of CRMs and transcription factor binding sites (TFBSs) ii) Identify genomic regions functionally active in the NSC through direct mapping of DNase I hypersensitivity sites. Putative functional annotation to these sites will be implemented and conduct direct CRM prediction of the identified regions iii) Identify motifs/signatures over-represented in distal elements found around NSC expressed genes iv) Identify the elements acting as transcriptional enhancers and define their pattern of activities in different neural cell lines and in vertebrate animal model systems v) Disseminate the results of the project to the research community, biotech/pharmaceutical companies and the general public

Field of science

  • /medical and health sciences/medical biotechnology/cells technologies/stem cells
  • /natural sciences/biological sciences/molecular biology

Call for proposal

FP7-HEALTH-2007-B
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
Address
Rue De L'universite 147
75338 Paris Cedex 07
France
Activity type
Research Organisations
EU contribution
€ 455 282
Administrative Contact
Jean-Stéphane Joly (Dr.)

Participants (8)

KARLSRUHER INSTITUT FUER TECHNOLOGIE
Germany
EU contribution
€ 441 749
Address
Kaiserstrasse 12
76131 Karlsruhe
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Natascha Wallburg (Ms.)
UNIVERSITA' DEGLI STUDI DI MILANO-BICOCCA
Italy
EU contribution
€ 353 360
Address
Piazza Dell'ateneo Nuovo 1
20126 Milano
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Anastasia Sguera (Dr.)
EUROPEAN MOLECULAR BIOLOGY LABORATORY
Germany
EU contribution
€ 672 800
Address
Meyerhofstrasse 1
69117 Heidelberg
Activity type
Research Organisations
Administrative Contact
Phil Irving (Dr.)
THE UNIVERSITY OF MANCHESTER
United Kingdom
EU contribution
€ 186 644
Address
Oxford Road
M13 9PL Manchester
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Liz Fay (Ms.)
RUPRECHT-KARLS-UNIVERSITAET HEIDELBERG
Germany
EU contribution
€ 357 300
Address
Seminarstrasse 2
69117 Heidelberg
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Laurence Ettwiller (Dr.)
MEDICAL RESEARCH COUNCIL
United Kingdom
EU contribution
€ 223 381
Address
North Star Avenue Polaris House 2 Floor David Phillips Building
SN2 1FL Swindon
Activity type
Research Organisations
Administrative Contact
Michele Marron (Ms.)
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
France
EU contribution
€ 175 560
Address
Rue Michel Ange 3
75794 Paris
Activity type
Research Organisations
Administrative Contact
Michele Saumon (Ms.)
INRAE TRANSFERT SAS
France
EU contribution
€ 118 093
Address
Rue Du Docteur Finlay 28
75015 Paris
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Lucie Hofmann (Ms.)