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Effective redesign of oxidative enzymes for green chemistry

Effective redesign of oxidative enzymes for green chemistry

Objective

Enzymes are extremely powerful natural catalysts able to perform almost any type of chemical reaction while being mild by nature and highly specific. In fact, the delicate functioning of enzymes forms the basis of every living creature. The catalytic potential of enzymes is more and more appreciated by the industry as many industrial processes rely on these sophisticated catalysts. However, the number of reactions catalyzed by enzymes is restricted as enzymes only have evolved to catalyze reactions that are physiologically relevant. Furthermore, enzymes have adapted to the direct (cellular) environment in which they have to function (e.g. operative at ambient temperature, resilient towards proteolysis, catalytic turnover rate should fit with metabolic enzyme partners). This excludes the existence of enzymes that do not fit within boundaries set by nature. It is a great challenge to go beyond these natural boundaries and develop methodologies to design ‘unnatural’ tailor-made enzymes. Ideally it should become possible to (re)design enzymes to convert pre-defined substrates. Such designer enzymes could theoretically exhibit unsurpassed catalytic properties and, obviously, will be of significant interest for industrial biotechnology. The OXYGREEN project aims at the design and construction of novel oxygenating enzymes (designer oxygenases) for the production of compounds that can be used in medicine, food and agriculture and the development of novel powerful and generic enzyme redesign tools for this purpose. The enzymes and whole-cell biocatalysts that will be developed should catalyze the specific incorporation of oxygen to afford synthesis of bioactive compounds in a selective and clean way, with minimal side products and with no use of toxic materials. For this, generic platform technologies (novel high-throughput methodology and methods for engineering dedicated host cells) will be developed that allow effective structure-inspired directed evolution of enzyme.

Coordinator

RIJKSUNIVERSITEIT GRONINGEN

Address

Broerstraat 5
9712cp Groningen

Netherlands

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 1 203 149

Administrative Contact

Jan Poutsma (Mr.)

Participants (13)

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JACOBS UNIVERSITY BREMEN GGMBH

Germany

EU Contribution

€ 121 989

TECHNISCHE UNIVERSITAT DORTMUND

Germany

EU Contribution

€ 882 950

BIOINFOBANK INSTITUTE

Poland

EU Contribution

€ 409 200

TECHNISCHE UNIVERSITAET WIEN

Austria

EU Contribution

€ 363 600

TECHNISCHE UNIVERSITAET GRAZ

Austria

EU Contribution

€ 832 013

UNIVERSITA DEGLI STUDI DI PAVIA

Italy

EU Contribution

€ 373 350

DSM RESEARCH B.V.

Netherlands

EU Contribution

€ 11 892

EVONIK REXIM

France

EU Contribution

€ 91 528

BIOLOG LIFE SCIENCE INSTITUTE, FORSCHUNGSLABOR UND BIOCHEMICA- VERTRIEB GMBH

Germany

EU Contribution

€ 258 400

ENZYSCREEN

Netherlands

EU Contribution

€ 98 400

DECHEMA GESELLSCHAFT FUER CHEMISCHE TECHNIK UND BIOTECHNOLOGIE E.V.

Germany

EU Contribution

€ 250 140

RHEINISCH-WESTFAELISCHE TECHNISCHE HOCHSCHULE AACHEN

Germany

EU Contribution

€ 578 530

DSM INNOVATIVE SYNTHESIS BV

Netherlands

EU Contribution

€ 68 108

Project information

Grant agreement ID: 212281

Status

Closed project

  • Start date

    1 May 2008

  • End date

    30 April 2013

Funded under:

FP7-KBBE

  • Overall budget:

    € 7 435 684,45

  • EU contribution

    € 5 543 249

Coordinated by:

RIJKSUNIVERSITEIT GRONINGEN

Netherlands