Objective An important question of modern neurobiology is how neurons regulate synaptic function in response to excitation. In particular, the roles of alternative pre-mRNA splicing and mRNA translation regulation in this response are poorly understood. We will study the RNA-binding proteins (RBPs) that control these post-transcriptional changes using a UV crosslinking-based purification method (CLIP) and ultra-high throughput sequencing. Computational analysis of the resulting data will define the sequence and structural features of RNA motifs recognized by each RBP. Splicing microarrays and translation reporter assays will then allow us to examine the regulatory functions of RBPs and RNA motifs. By integrating the biochemical and functional datasets, we will relate the position of RNA motifs to the activity of bound RBPs, and predict the interactions that act as central nodes in the regulatory network. The physiological role of these core RBP-RNA interactions will then be tested using antisense RNAs. Together, these projects will provide insights to the regulatory mechanisms underlying neuronal activity-dependent changes, and provide new opportunities for future treatments of neurodegenerative disorders. Fields of science natural sciencesbiological sciencesneurobiologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticsRNA Keywords RNA-protein interactions activity-dependent neuronal responses alternative splicing antisense RNA technology mRNA translation Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) ERC-SG-LS1 - ERC Starting Grant - Molecular and Structural Biology and Biochemistry Call for proposal ERC-2007-StG See other projects for this call Funding Scheme ERC-SG - ERC Starting Grant Host institution UNIVERSITY COLLEGE LONDON EU contribution € 80 923,20 Address GOWER STREET WC1E 6BT London United Kingdom See on map Region London Inner London — West Camden and City of London Activity type Higher or Secondary Education Establishments Principal investigator Jernej Ule (Dr.) Administrative Contact Malgorzata Kielbasa (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Beneficiaries (3) Sort alphabetically Sort by EU Contribution Expand all Collapse all UNIVERSITY COLLEGE LONDON United Kingdom EU contribution € 80 923,20 Address GOWER STREET WC1E 6BT London See on map Region London Inner London — West Camden and City of London Activity type Higher or Secondary Education Establishments Principal investigator Jernej Ule (Dr.) Administrative Contact Malgorzata Kielbasa (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data UNIVERZA V LJUBLJANI Slovenia EU contribution € 144 000,00 Address KONGRESNI TRG 12 1000 Ljubljana See on map Region Slovenija Zahodna Slovenija Osrednjeslovenska Activity type Higher or Secondary Education Establishments Administrative Contact Jasna Bevk (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data MEDICAL RESEARCH COUNCIL Participation ended United Kingdom EU contribution € 675 076,80 Address 20 Park Crescent W1B 1AL LONDON See on map Activity type Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments) Administrative Contact Elizabeth Cutler (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
