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Targeted DISCOvery of novel cellulases and hemicellulases and their reaction mechanisms for hydrolysis of lignocellulosic biomass

Objective

The aim of the proposed DISCO project is to develop more efficient and therefore more cost-effective cellulosic and hemicellulosic enzyme tools for the enhanced hydrolysis of pre-treated lignocellulosic biomass in simultaneous saccharification and fermentation (SSF) conditions for bioethanol production. The focus will be on enzymes having increased catalytic activity on various types of relevant European lignocellulosic biomass. In addition enzymes with lower affinity for lignin shall also be developed. Such enzymes would increase the effective amount of cellulases/ hemicellulases for cellulose hydrolysis. Furthermore the recycling of these enzymes would be applicable. The approach in this proposal is to discover the desired activities by combining classical and modern screening technologies. The enzymes will be produced in suitable host systems for industrial enzyme production. The project also focuses on elucidation of enzymatic hydrolysis mechanisms, about which there is a paucity of knowledge. The project will determine the limiting structural factors in these mechanisms by characterisation of the substrate during the course of the hydrolysis and the remaining recalcitrant residue. Synergy between different cellulase and hemicellulases components will also be addresses on the chosen lignocellulosic substrates. Furthermore, the project seeks to demonstrate the proof of concept with the cellulolytic enzymes in a pilot scale using the most relevant European feedstock pretreated wheat straw and related high-volume co-products.

Field of science

  • /engineering and technology/industrial biotechnology/bioprocessing technologies/fermentation
  • /engineering and technology/environmental engineering/waste management/recycling
  • /agricultural sciences/agricultural biotechnology/biomass
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes

Call for proposal

FP7-KBBE-2007-1
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

TEKNOLOGIAN TUTKIMUSKESKUS VTT
Address
Tekniikantie 4 A
02044 VTT Espoo
Finland
Activity type
Research Organisations
EU contribution
€ 800 000
Administrative Contact
Riitta Kervinen (Ms.)

Participants (11)

COOPERATIE CEHAVE LANDBOUWBELANG U.A.

Participation ended

Netherlands
EU contribution
€ 21 500
Address
Poort Van Veghel 4949
5466 SB Veghel
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Albert Jan Van Dijk (Dr.)
WAGENINGEN UNIVERSITY
Netherlands
EU contribution
€ 395 505
Address
Droevendaalsesteeg 4
6708 PB Wageningen
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Jos Teunissen (Mr.)
QUADRAM INSTITUTE BIOSCIENCE
United Kingdom
EU contribution
€ 395 000
Address
Quadram Institute Bioscience Norwich Research Park
NR4 7UQ Norwich
Activity type
Research Organisations
Administrative Contact
Mary Anderson (Dr.)
HELSINGIN YLIOPISTO
Finland
EU contribution
€ 413 400
Address
Yliopistonkatu 3
00014 Helsingin Yliopisto
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Sanna-Maija Miettinen (Dr.)
A.N. BAKH INSTITUTE OF BIOCHEMISTRY OF THE RUSSIAN ACADEMY OF SCIENCES
Russia
EU contribution
€ 225 000
Address
Leninsky Prospect 33
119071 Moscow
Activity type
Research Organisations
Administrative Contact
Vladimir Popov (Prof.)
BUDAPESTI MUSZAKI ES GAZDASAGTUDOMANYI EGYETEM
Hungary
EU contribution
€ 189 750
Address
Muegyetem Rakpart 3
1111 Budapest
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Miklós Zrínyi (Prof.)
DYADIC NEDERLAND BV
Netherlands
EU contribution
€ 328 190
Address
Nieuwe Kanaal 7 S
6709 PA Wageningen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Wim Van Der Wilden (Dr.)
BIOGOLD OU
Estonia
EU contribution
€ 97 500
Address
Lossi 19A
12616 Tallinn
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Priit Kotli (Mr.)
G R WRIGHT AND SONS LTD
United Kingdom
EU contribution
€ 25 000
Address
Ponders End Mills
EN3 4Tg Enfield
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Lesley Morris (Ms.)
SEKAB E-TECHNOLOGY AB
Sweden
EU contribution
€ 95 000
Address
Hoerneborgsvaegen
89121 Oernskoeldsvik
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Torbjörn Van Der Meulen (Dr.)
AGRIFIRM GROUP BV
Netherlands
Address
Landgoedlaan 20
7325 AW Apeldoorn
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Erik Langenhuijzen (Mr.)