Obiettivo Reproduction is a complex process governed by the interplay of central and peripheral factors. The major signal controlling reproduction is the hypothalamic GnRH, responsible of the release of pituitary gonadotropins that drive gonadal function. Thus, diverse conditions disturbing reproductive function are related to defects in factors and/or routes involved in the control of GnRH release. As an example, kisspeptins (encoded by the Kiss1 gene) and their receptor GPR54 have emerged as essential gatekeepers of GnRH neurons, and disruption of kisspeptin/GPR54 signaling lead to hypogonadotropic hypogonadism (HH) in humans and mice (one of the most relevant breakthroughs in contemporary Neuroendocrinology). However, the mechanisms that regulate the hypothalamic Kiss1 system remain partially unknown. Recently, prokineticin-2 (PK2) and its receptor (PKR2) have been involved in the control of reproductive maturation and/or function, as loss-of-function mutations in either the receptor or the ligand lead to HH. Considering that PK2 was originally described as a circadian pacemaker from the suprachiasmatic nucleus (SCN; the central translator of the photoperiodic status to the organism) and that key aspects of reproductive function (such as the preovulatory LH surge; responsible for ovulation) are exquisitely timed, the AIM of this project is to explore the basis for the regulatory actions of the PK2/PK2R system on GnRH neurons, and to evaluate whether Kiss1 neurons operate as essential relay for such a transmission. To accomplish this goal, a combination of gene expression (RT-PCR, in situ hybridization), protein (ICC) assays and functional (hormonal) tests, will be implemented in wild-type and genetically-modified mice. Implementation of this project will broaden our basic knowledge on the physiological mechanisms governing reproduction in mammals; knowledge which might be translatable to rational therapeutic strategies for the control (promotion or blockade) of fertility. Campo scientifico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinssocial sciencessociologydemographyfertilitynatural sciencesbiological sciencesgeneticsmutationnatural sciencesbiological scienceszoologymammalogy Parole chiave GnRH Health sciences Neurophysiology PK2/PK2R kisspeptins reproduction Programma(i) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) PEOPLE-2007-4-1.IOF - Marie Curie Action: "International Outgoing Fellowships for Career Development" Invito a presentare proposte FP7-PEOPLE-2007-4-1-IOF Vedi altri progetti per questo bando Meccanismo di finanziamento MC-IOF - International Outgoing Fellowships (IOF) Coordinatore UNIVERSIDAD DE CORDOBA Contributo UE € 225 998,97 Indirizzo AVENIDA DE MEDINA AZAHARA 5 14005 Cordoba Spagna Mostra sulla mappa Regione Sur Andalucía Córdoba Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Antonia López Navajas (Ms.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato