In vitro reconstitution of the S. pombe interphase microtubule cytoskeleton

Projektinformationen

PLUSTIPS

ID Finanzhilfevereinbarung: 221455

Abgeschlossenes Projekt

Startdatum 1 Mai 2008

Enddatum 30 April 2010

Finanziert unter

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Gesamtkosten € 158 694,85

EU-Beitrag € 158 694,85

Koordiniert durch

EUROPEAN MOLECULAR BIOLOGY LABORATORY
Germany

Ziel

The research proposed here intends to explore processes governed by microtubule (MT) plus end accumulating proteins (+TIPs) and interdependencies between these proteins for their localisation. The well defined but relatively simple interphase MT cytoskeleton of Schitzosaccharomyces pombe (S. pombe) has proven to be an excellent model for MT plus end studies. In interphase, MTs are involved in defining sites of polarised growth as well as nuclear positioning by generating pushing forces against the cell cortex. The mechanochemical principles governing this process as well as the likely interplay between +TIPs are as yet incompletely understood. This proposal focuses on elucidating the interdependencies between the various evolutionary conserved +TIPs using (purified proteins of) S. pombe in both in vivo and in vitro experiments. Initial experiments will determine the function of the recently characterised class of length-dependent MT-depolymerases (kinesin-8s) in MT plus end behaviour in S. pombe. Combined with already characterised +TIPs such as Mal3, Tip1, and Tea2, a complete set of proteins, governing all observed MT dynamic behaviours seen in vivo, may now be available. These will be used in an attempt to recreate the first fully functional MT plus end in vitro. Expanding on a novel microscopy set-up created by the Surrey laboratory (EMBL, Heidelberg, Germany, unpublished) we hope to determine the interplay between +TIPs for MT plus end localisation. In addition, we want to investigate the individual as well as the combined influence of these +TIPs on MT dynamic behaviour in vitro. Skills acquired during this research at the interphase of cell biology and biophysics using both in vitro and in vivo techniques will be highly advantageous in obtaining an independent position after the fellowship in these developing fields of biological research.

Wissenschaftliches Gebiet

• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• natural sciences › biological sciences › cell biology
• natural sciences › physical sciences › optics › microscopy
• natural sciences › biological sciences › biophysics

Schlüsselbegriffe

• +TIPs
• Cell biology
• Cytology
• Kinesins
• Klp5
• Klp6

Programm/Programme

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Thema/Themen

• PEOPLE-2007-2-1.IEF - Marie Curie Action: "Intra-European Fellowships for Career Development"

Aufforderung zur Vorschlagseinreichung

FP7-PEOPLE-2007-2-1-IEF
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

MC-IEF - Intra-European Fellowships (IEF)

Koordinator

EUROPEAN MOLECULAR BIOLOGY LABORATORY

Kontakt Verwaltung

Damian Brunner (Dr.)

• EU-Beitrag: € 158 694,85
• Adresse: Meyerhofstrasse 1 ; 69117 Heidelberg ; Deutschland
• Region: Baden-Württemberg › Karlsruhe › Heidelberg, Stadtkreis
• Aktivitätstyp: Research Organisations
• Gesamtkosten: Keine Daten