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Coordination of resources for conditional expression of mutated mouse alleles

Objective

This proposal describes CREATE, an internationally based Coordination Action directly addressing a large scale data gathering activity for systematic conditional mouse mutagenesis. Advances in the sophisticated manipulation of the mouse genome has established the mouse as the premier organism for developing models of human disease. Internationally coordinated initiatives have established for the systematic generation of conditional mouse mutants on a large scale employing the Cre-lox binary system. Production of mutant mouse ES cell lines, each of which carries an altered or “floxed” allele of a single gene allows the creation of somatic mutations in defined genes. The full power of conditional mutants can only be exploited with the availability of well characterised mouse lines expressing Cre-recombinase in tissue, organ and cell type-specific patterns. Although several privately curated and locally held databases currently provide a limited catalog of existing Cre driver strains, these are not well integrated, and published data on Cre driver mice do not always capture critical details. CREATE will address these these combined shortcomings - inaccessibility to existing Cre driver strains, their incomplete characterisation, and an inadequate coverage of cell and tissue types in which they are active – which will soon constitute the major bottleneck for the future development of targeted conditional mutant models for the worldwide mouse mutagenesis initiative. Its main deliverables will be a well-annotated and integrated data resource of Cre driver strains, and coordination of the expansion of the resource, both necessary components of the international mouse mutagenesis effort. By enlisting major mouse stakeholders dedicated to collecting, integrating and curating, expanding and disseminating Cre driver mouse databases through a unified web portal, CREATE will provide the necessary structure for worldwide access to these critical resources.

Field of science

  • /natural sciences/computer and information sciences/databases

Call for proposal

FP7-HEALTH-2007-B
See other projects for this call

Funding Scheme

CSA-CA - Coordination (or networking) actions

Coordinator

EUROPEAN MOLECULAR BIOLOGY LABORATORY
Address
Meyerhofstrasse 1
69117 Heidelberg
Germany
Activity type
Research Organisations
EU contribution
€ 349 750
Administrative Contact
Keith Williamson (Mr.)

Participants (7)

BIOMEDICAL SCIENCES RESEARCH CENTER ALEXANDER FLEMING
Greece
EU contribution
€ 294 250
Address
Fleming Street 34
16672 Vari-athens
Activity type
Research Organisations
Administrative Contact
George Kollias (Dr.)
CENTRE EUROPEEN DE RECHERCHE EN BIOLOGIE ET MEDECINE
France
EU contribution
€ 155 274
Address
Rue Laurent Fries 1
67404 Illkirch Graffenstaden
Activity type
Research Organisations
Administrative Contact
Steve Brooks (Dr.)
HELMHOLTZ ZENTRUM MUENCHEN DEUTSCHES FORSCHUNGSZENTRUM FUER GESUNDHEIT UND UMWELT GMBH
Germany
EU contribution
€ 45 475
Address
Ingolstadter Landstrasse 1
85764 Neuherberg
Activity type
Research Organisations
Administrative Contact
Juergen Ertel (Dr.)
MEDICAL RESEARCH COUNCIL
United Kingdom
EU contribution
€ 42 000
Address
North Star Avenue Polaris House 2 Floor David Phillips Building
SN2 1FL Swindon
Activity type
Research Organisations
Administrative Contact
Hilary Gates (Dr.)
GENOME RESEARCH LIMITED
United Kingdom
EU contribution
€ 42 051
Address
The Gibbs Building, Euston Road 215
NW1 2BE London
Activity type
Research Organisations
Administrative Contact
David Neil Davison (Mr.)
Mount Sinai Hospital
Canada
EU contribution
€ 85 600
Address
University Avenue 600
M5G 1X5 Toronto
Activity type
Research Organisations
Administrative Contact
Paul Kranjac (Mr.)
THE JACKSON LABORATORY NON PROFIT CORPORATION
United States
EU contribution
€ 85 600
Address
Main Street 610
04609 Bar Harbor
Activity type
Other
Administrative Contact
Sven Davisson (Dr.)