Objetivo The most important prerequisite and challenge in structural biology research at the atomic level by any method is the availability of sufficiently large amounts of highly purified functional proteins due to the increasing size and complexity of target proteins or protein complexes to be analyzed. Heterologous expression in bacteria, yeast, insect, or mammalian cells combined with many attempts involving different DNA constructs that result in a large variety of protein variants is instrumental to obtain sufficiently high yields. This means high-throughput methods employing robotics and specialized infrastructure are essential to efficiently reach the state of obtaining functional protein and/or crystals for X-ray structure determination. PCUBE ('protein production platform') combines existing infrastructures and know-how of leading European laboratories in bacterial, eukaryotic expression of proteins, in high-throughput crystallization and in libraries design for the effective production and crystallization of macromolecules. The program aims at offering these various state-of-the-art platforms to research groups from EC member and associated states for the efficient production of proteins for their structural studies. Each infrastructure will offer defined procedures for applying for transnational access. Scientific selection committees will give priorities to applications by scientific criteria of excellence only. Researchers from the different sites offering infrastructure are combining efforts to improve the methods in the various areas by collaborating by joint research activities. These include the improvement of the automation for synthesising DNA constructs, for parallel expression, for improved libraries, for the efficient selection of affinity molecules from libraries to particular target proteins as well as developing new methods for crystallization at the nanoliter scale. Ámbito científico natural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological scienceszoologyentomologynatural sciencesbiological sciencesmolecular biologystructural biology Palabras clave ANK-SEL DNA- and ankyrin libraries ESPRIT FLPAL HT-X MultiBac OPPF capillary crystallisation fluorescence labelling high thoughput protein crystallisation in situ imaging protein expression Programa(s) FP7-INFRASTRUCTURES - Specific Programme "Capacities": Research infrastructures Tema(s) INFRA-2008-1.1.1 - Bottom-up approach: Integrating Activities in all scientific and technological fields Convocatoria de propuestas FP7-INFRASTRUCTURES-2008-1 Consulte otros proyectos de esta convocatoria Régimen de financiación CP-CSA-Infra - Combination of CP and CSA Coordinador University of Zurich Aportación de la UE € 1 730 245,00 Dirección RAMISTRASSE 71 8006 ZURICH Suiza Ver en el mapa Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Markus, G Gruetter (Prof.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Participantes (2) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Reino Unido Aportación de la UE € 1 649 749,00 Dirección WELLINGTON SQUARE UNIVERSITY OFFICES OX1 2JD Oxford Ver en el mapa Región South East (England) Berkshire, Buckinghamshire and Oxfordshire Oxfordshire Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Susan Daenke (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos EUROPEAN MOLECULAR BIOLOGY LABORATORY Alemania Aportación de la UE € 3 220 003,00 Dirección Meyerhofstrasse 1 69117 Heidelberg Ver en el mapa Región Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis Tipo de actividad Research Organisations Contacto administrativo Keith Williamson (Mr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos